Recombinant Human IL7R protein (Met1-Gly236, I66T, V138I), His-tagged

• Cat.No.: IL7R-2261H
• Product Overview: Recombinant Human IL7R protein(P16871-1) (Met1-Gly236, with mutations I66T, V138I) was expressed in HEK293 with a polyhistidine tag at the C-terminus.

Specification

• Source: HEK293
• Species: Human
• Tag: His
• Protein length: Met1-Gly236, I66T, V138I
• Form: Lyophilized from sterile PBS, pH 7.4
• Bio-activity: Measured by its ability to bind biotinylated mouse TSLP-his in functional ELISA.
• Molecular Mass: The recombinant human IL7Rαconsists of 227 amino acids and has a predicted molecular mass of 26.3 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of human IL7Rα is approximately 44 kDa due to glycosylation.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: ≥ 92 % as determined by SDS-PAGE. ≥ 95 % as determined by SEC-HPLC.
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: IL7R interleukin 7 receptor [ Homo sapiens ]
• Official Symbol: IL7R
• Synonyms: IL7R; interleukin 7 receptor; interleukin-7 receptor subunit alpha; CD127; IL-7RA; CD127 antigen; IL-7R subunit alpha; IL-7 receptor subunit alpha; interleukin 7 receptor alpha chain; interleukin 7 receptor isoform H5-6; ILRA; IL7RA; CDW127; IL-7R-alpha
• Gene ID: 3575
• mRNA Refseq: NM_002185
• Protein Refseq: NP_002176
• MIM: 146661
• UniProt ID: P16871