Recombinant Human IL3, His-tagged

• Cat.No.: IL3-56H
• Product Overview: Recombinant Human Interleukin-3 produced inE.Coliis single, a non-glycosylated, Polypeptide chain containing 133 amino acids fragment (20-152) and having a total molecular mass of 15 kDa with an amino-terminal hexahistidine tag. The IL3 His is purified by proprietary chromatographic techniques.

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Protein Length: 20-152 a.a.
• Description: Interleukin-3 is a pleiotropic cytokine produced primarily by activated T cells. IL-3 is thought to function via specific cell surface receptors to stimulate the proliferation, differentiation and survival of haematopoietic cell lines. IL-3 has also been shown to affect the functional activity of a variety of other cell types including mast cells, eosinophils, megakaryocytes and basophils.
• Physical Appearance: Sterile Filtered clear solution.
• Purity: Greater than 95.0% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
• Formulation: Interleukin-3 His is supplied in 1x PBS and 50% glycerol.
• Biological activity: The ED50 for this effect is<0.53 ng/ml="" .="" measured="" in="" a="" cell="" proliferation="" assayusing="" tf1="" human="" erythroleukemic="">
• Activity Assay: 1.Cell line: TF1 (human erythroleukemic cell)2.Maintenance Condition: 10% FBS RPMI 1640 with GM-CSF3.Assay Medium: 10% FBS RPMI 1640 without GM-CSF4.Cell Density: 2 x 104 cells/well (96 well plate, final volume 100ul)5.Starvation : 24hr, Assay medium6.Incubation Time : 48 hr (after sample treatment)7.Concentration Range: 0.048ng/ml - 25ng/ml 8.Detection method : MTT assay
• Storage: Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. Please avoid freeze thaw cycles.

Gene Information

• Gene Name: IL3 interleukin 3 (colony-stimulating factor, multiple) [ Homo sapiens ]
• Synonyms: IL3; interleukin 3 (colony-stimulating factor, multiple); IL-3; MCGF; MGC79398; MGC79399; MULTI-CSF; interleukin 3; mast-cell growth factor; P-cell stimulating factor; hematopoietic growth factor; Mast cell growth factor; multilineage-colony-stimulating factor; Multipotential colony-stimulating factor; Hematopoietic growth factor
• Gene ID: 3562
• mRNA Refseq: NM_000588
• Protein Refseq: NP_000579
• MIM: 147740
• UniProt ID: P08700
• Chromosome Location: 5q31.1
• Pathway: Cytokine-cytokine receptor interaction; Fc epsilon RI signaling pathway; Hematopoietic cell lineage; Jak-STAT signaling pathway; Apoptosis; Asthma
• Function: cytokine activity; growth factor activity; protein binding; interleukin-3 receptor binding