Recombinant Human IL23R protein(Met1-Asp353), hFc-tagged

• Cat.No.: IL23R-2400H
• Product Overview: Recombinant Human IL23R (AAM44229.1) (Met1-Asp353) was expressed with the Fc region of Human IgG1 at the C-terminus.

Specification

• Species: Human
• Source: HEK293
• Tag: Fc
• Protein Length: Met1-Asp353
• Form: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Bio-activity: Measured by its binding ability in a functional ELISA.1. Immobilized human IL23A-His+IL12B-His at 10 μg/ml (100 μl/well) can bind human IL23R-Fc. The EC50 of human IL23R-Fc is 0.28-0.66 μg/ml. 2. Immobilized mouse IL12Bh (m)+mIL23Ah at 10 μg/ml (100 μl/well) can bind human IL23R-Fc. The EC50 of human IL23R-Fc is 0.14-0.35 μg/ml.
• Molecular Mass: The recombinant human IL23R /Fc is a disulfide-linked homodimer. The reduced monomer comprises 571 amino acids and has a predicted molecular mass of 64.9 kDa. The apparent molecular mass of the protein is approximately 92 kDa in SDS-PAGE under reducing conditions due to glycosylation.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 90 % as determined by SDS-PAGE. ≥ 95 % as determined by SEC-HPLC.
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: IL23R interleukin 23 receptor [ Homo sapiens ]
• Official Symbol: IL23R
• Synonyms: IL23R; interleukin 23 receptor; interleukin-23 receptor; IL 23R; IL-23 receptor
• Gene ID: 149233
• mRNA Refseq: NM_144701
• Protein Refseq: NP_653302
• MIM: 607562
• UniProt ID: Q5VWK5