Recombinant Human IL2 protein

• Cat.No.: IL2-06H
• Product Overview: Recombinant Human IL2 protein(P60568) was expressed in E. coli.

Specification

• Source: E. coli
• Species: Human
• Tag: Non
• Protein length: 133 amino acids
• Form: Lyophilized from a 0.2 μm filtered concentrated solution in 20 mM PB, pH 3.5.
• Bio-activity: Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using murine CTLL-2 cells is less than 0.1 ng/ml, corresponding to a specific activity of > 1.0 × 10^7 IU/mg.
• Molecular Mass: Approximately 15.4 kDa, a single non-glycosylated polypeptide chain containing 133 amino acids.
• AASequence: APTSSSTKKT QLQLEHLLLD LQMILNGINN YKNPKLTRML TFKFYMPKKA TELKHLQCLE EELKPLEEVL NLAQSKNFHL RPRDLISNIN VIVLELKGSE TTFMCEYADE TATIVEFLNR WITFCQSIIS TLT
• Endotoxin: Less than 1.0 EU/μg of rHuIL-2 as determined by LAL method.
• Purity: > 97 % by SDS-PAGE and HPLC analyses.
• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  - 12 months from date of receipt, -20 to -70 °C as supplied.
  - 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  - 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C.

Gene Information

• Gene Name: IL2 interleukin 2 [ Homo sapiens ]
• Official Symbol: IL2
• Synonyms: IL2; interleukin 2; interleukin-2; IL 2; T cell growth factor; TCGF; aldesleukin; involved in regulation of T-cell clonal expansion; IL-2; lymphokine
• Gene ID: 3558
• mRNA Refseq: NM_000586
• Protein Refseq: NP_000577
• MIM: 147680
• UniProt ID: P60568