Recombinant Human IL2-inducible T-cell Kinase, GST-tagged, Active

• Cat.No.: ITK-335H
• Product Overview: Recombinant human ITK (352-end) was expressed by baculovirus inSf9 insect cellsusing an N-terminal GST tag. MW = 53 kDa.

Specification

• Species: Human
• Source: Sf9 Cells
• Tag: GST
• ProteinLength: 352-end a.a.
• Description: ITK is a member of the TEC family of non-receptor tyrosine kinases. ITK is expressed in T-cells and is important for T-cell development and activation through the antigen receptor. ITK require prior activation of Lck, Zap-70 and PI3-kinase for efficient activation and shares major substrates with both Lck and Zap-70. ITK knockout mice show multiple effects on T cell development, cytokine production and T-helper cell differentiation. T cells that lack or express mutant versions of ITK show impaired TCR-induced actin polymerization, cell polarization and regulation of the signaling events involved in cytoskeletal reorganization.
• Sequence: 352-end.
• Applications: Kinase Assay, Western Blot.
• Storage And Stability: Store product at -70℃. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.

Gene Information

• Gene Name: ITK IL2-inducible T-cell kinase [ Homo sapiens ]
• Synonyms: ITK; IL2-inducible T-cell kinase; EMT; LYK; PSCTK2; MGC126257; MGC126258; tyrosine-protein kinase LYK; homolog of mouse T-cell itk/tsk; tyrosine-protein kinase ITK/TSK; EC 2.7.10.2; Kinase EMT
• Gene ID: 3702
• mRNA Refseq: NM_005546
• Protein Refseq: NP_005537
• MIM: 186973
• UniProt ID: Q08881
• Chromosome Location: 5q31-q32
• Pathway: Chemokine signaling pathway; Leukocyte transendothelial migration; T cell receptor signaling pathway
• Function: ATP binding; metal ion binding; non-membrane spanning protein tyrosine kinase activity; nucleotide binding; protein binding; transferase activity; zinc ion binding