Recombinant Human IL1F10

• Cat.No.: IL1F10-319H
• Product Overview: Recombinant Human IL-1F10 produced in E.coli is a single, non-glycosylated, polypeptide chain containing 152 amino acids and having a molecular mass of 17 kDa.

Specification

• Cat. No.: IL1F10-319H
• Description: Human interleukin family 1, member 10 (hIL-1F10 / FIL1-theta) is a member of the interleukin 1 cytokine family. IL-1F10 was expressed in fetal skin, spleen and tonsil,mostly in the basal epithelia of skin and in proliferating B-cells of the tonsil. IL-1F10 binds soluble IL-1 receptor type 1 and may be implicated in regulating adapted and innate immune responses. Two alternatively spliced transcript variants encoding the same protein have been reported.
• Biological Activity: As measured by its binding ability in a functional ELISA, immobilized rHuIL-1F10 at 1 μg/ml (100 μl/well) can bind rHuIL-1 Rrp2/Fc Chimera with a linear range of 0.15~5 μg/ml.
• Purity: Greater than 95% as determined by (a) Analysis by RP-HPLC; (b) Analysis by reducing and non-reducing SDS-PAGE Silver Stained gel.
• Amino-Acid Sequence: The sequence of the first five N-terminal amino acids was determined and was found to be Met-Cys-Ser-Leu-Pro.
• Endotoxin: Less than 0.1ng/µg (1 IEU/µg) determined by LAL test.
• Form: The protein was lyophilized after extensive dialysis against 20mM Phosphate buffer, pH7.4.
• Reconstitution: It is recommended to quick spin followed by reconstitution of rHuIL-1F10 in PBS to a concentration no less than 100µg/ml, which can then be further diluted to other aqueous solutions.
• Storage: Lyophilized rHuIL-1F5 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution rHuIL-1F10 should be stored at 4°C between 2-7 days and for future use below -18°C. For long-term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please avoid freeze-thaw cycles.
• Tag: Non

Gene Information

• Gene Name: IL1F10 interleukin 1 family, member 10 (theta) [ Homo sapiens ]
• Official Symbol: IL1F10
• Synonyms: IL1F10; interleukin 1 family, member 10 (theta); FKSG75; IL-1HY2; IL1-theta; MGC119831; MGC1198- 32; MGC119833; FIL1-theta; interleukin-1 family member 10; FIL1 theta; IL-1 theta; interleukin-1 HY2; OTTHUMP00000162018; OTTHUMP00000203- 696; interleukin-1 theta; IL-1F10 (canonical form IL-1F10a); interleukin-1 receptor antagonist FKSG75; interleukin-1 receptor antagonist-like FIL1 theta; Interleukin-1 theta; Interleukin-1 HY2; FIL1T
• Gene ID: 84639
• mRNA Refseq: NM_032556
• Protein Refseq: NP_115945
• UniProt ID: Q8WWZ1
• Chromosome Location: 2q13
• Function: cytokine activity; interleukin-1 receptor antagonist activity