Recombinant Human IL17A & IL17F protein, His-tagged

• Cat.No.: IL17A & IL17F-1258H
• Product Overview: Recombinant Human IL17A & IL17F protein(Q16552-1(IL-17A)&Q96PD4(IL-17F))(Gly24-Ala155(IL17A) & Arg31-Gln163(IL17F)), fused with C-terminal His tag, was expressed in HEK293.

Specification

• Species: Human
• Source: HEK293
• Tag: His
• ProteinLength: Gly24-Ala155(IL17A) & Arg31-Gln163(IL17F)
• Tag: C-His
• Form: Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization.
• Bio-activity: Immobilized Human IL-17A&F, His Tag at 5μg/ml (100μl/well) on the plate. Dose response curve for Anti-IL-17A Antibody, hFc Tag with the EC50 of 1.6ng/ml determined by ELISA.
• Molecular Mass: The protein has a predicted MW of 15.1 kDa (IL-17A) & 15.7 kDa (IL-17F). Due to glycosylation, the protein migrates to 16 kDa & 27 kDa based on Tris-Bis PAGE result.
• Endotoxin: Less than 1EU per μg by the LAL method.
• Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
• Storage: Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
• Reconstitution: Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
• GeneID 2: 112744

Gene Information

• Gene Name: IL17A interleukin 17A [ Homo sapiens ]
• Official Symbol: IL17A
• Synonyms: IL17A; interleukin 17A; CTLA8, IL17, interleukin 17 (cytotoxic T lymphocyte associated serine esterase 8); interleukin-17A; cytotoxic T lymphocyte associated protein 8; IL 17; IL 17A; CTLA-8; cytotoxic T-lymphocyte-associated antigen 8; cytotoxic T-lymphocyte-associated protein 8; cytotoxic T-lymphocyte-associated serine esterase 8; interleukin 17 (cytotoxic T-lymphocyte-associated serine esterase 8); IL17; CTLA8; IL-17; IL-17A
• Gene ID: 3605
• mRNA Refseq: NM_002190
• Protein Refseq: NP_002181
• MIM: 603149
• UniProt ID: Q16552
• Gene Name 2: IL17F interleukin 17F [ Homo sapiens ]
• Official Symbol 2: IL17F
• Synonyms 2: IL17F; interleukin 17F; interleukin-17F; IL 17F; ML 1; ML1; IL-24; cytokine ML-1; mutant IL-17F; interleukin-24; ML-1; CANDF6; IL-17F
• mRNA Refseq 2: NM_052872
• Protein Refseq 2: NP_443104
• MIM 2: 606496
• UniProt ID 2: Q96PD4