Recombinant Human IgG Fc Control Protein

• Cat.No.: IgG-02H
• Product Overview: Recombinant Human IgG Fc Control Protein was expressed in HEK293 Cells and purified using protein A-agarose.

Specification

• Species: Human
• Source: HEK293
• Tag: Non
• Description: Recombinatant fusion proteins consisting of the extracellular domain of immunoregulatory proteins and the constant (Fc) domain of immunoglobulin G (IgG) represent a growing class of human therapeutics. Chimeric protein of Human target protein and Human IgG-Fc domain can be purified using protein A chromatography.
• Predicted MolecularMass: ~ 55 kDa
• Formulation: 50µg of recombinant protein in 50µl of 1X PBS, 20%(v/v) glycerol. Frozen solution.
• Concentration: 1 mg/mL
• Purity: Greater than 90% by SDS-PAGE and Coomassie blue staining. Western blotting by primary antibody Mouse anti-Human IgG, clone HP6017, Fc and secondary antibody Goat anti-Mouse IgG, HRP conjugate
• Storage: Best stored at -80°C for up to 6 months after date of receipt. Thawed aliquots should be used immediately, as repeated freeze/thaw cycles might result in loss of activity.
• Introduction: Immunoglobulin G (IgG) is an antibody isotype. It is a protein complex composed of four peptide chains — two identical heavy chains and two identical light chains arranged in a Y-shape typical of antibody monomers. Each IgG has two antigen binding sites. Representing approximately 75% of serum immunoglobulins in humans, IgG is the most abundant antibody isotype found in the circulation. IgG molecules are synthesized and secreted by plasma B cells.
• Keywords: IgG Fc; IgG Fc(Human); Recombinant Human IgG Fc
• Publications:
  The neonatal Fc receptor (FcRn) binds independently to both sites of the IgG homodimer with identical affinity (2015)