Recombinant Human IGFBP3 protein, His-tagged

• Cat.No.: IGFBP3-828H
• Product Overview: Recombinant Human IGFBP3 aa. (Gly135~Thr277 (Accession # P17936)) fused with N-terminal His tag was produced in E. coli cells.

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Protein Length: Gly135~Thr277
• Description: This gene is a member of the insulin-like growth factor binding protein (IGFBP) family and encodes a protein with an IGFBP domain and a thyroglobulin type-I domain. The protein forms a ternary complex with insulin-like growth factor acid-labile subunit (IGFALS) and either insulin-like growth factor (IGF) I or II. In this form, it circulates in the plasma, prolonging the half-life of IGFs and altering their interaction with cell surface receptors. Alternate transcriptional splice variants, encoding different isoforms, have been characterized.
• Form: Freeze-dried powder
• Molecular Mass: Predicted Molecular Mass: 17.5kDa
• Endotoxin: <1.0EU per 1µg (determined by the LAL method)
• Purity: > 95%
• Characteristic: The isoelectric point is 9.3.
• Applications: SDS-PAGE; WB; ELISA; IP
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
• Storage buffer: Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4

Gene Information

• Gene Name: IGFBP3 insulin like growth factor binding protein 3 [ Homo sapiens (human) ]
• Official Symbol: IGFBP3
• Synonyms: IGFBP3; insulin like growth factor binding protein 3; IBP3; BP-53; insulin-like growth factor-binding protein 3; IBP-3; IGF-binding protein 3; IGFBP-3; acid stable subunit of the 140 K IGF complex; binding protein 29; binding protein 53; growth hormone-dependent binding protein
• Gene ID: 3486
• mRNA Refseq: NM_000598.4
• Protein Refseq: NP_000589.2
• UniProt ID: P17936