Recombinant Human IgE protein, His-Avi-tagged

• Cat.No.: IgE-1556H
• Product Overview: Recombinant Human IgE protein(P01854)(Cys209-Lys428), fused with C-terminal His and Avi tag, was expressed in HEK293.

Specification

• Source: HEK293
• Species: Human
• Tag: C-His-Avi
• Protein length: Cys209-Lys428
• Form: Supplied as 0.22μm filtered solution in 20mM PB, 500mM NaCl, 10% glycerol (pH 7.4).
• Bio-activity: Immobilized Human IgE, His Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Anti-IgE Antibody, hFc Tag with the EC50 of 7.0ng/ml determined by ELISA.
• Molecular Mass: The protein has a predicted MW of 27.8 kDa. Due to glycosylation, the protein migrates to 30-35 kDa based on Tris-Bis PAGE result.
• Endotoxin: Less than 1EU per μg by the LAL method.
• Purity: > 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC.
• Storage: Valid for 12 months from date of receipt when stored at -80°C.;Recommend to aliquot the protein into smaller quantities for optimal storage. Please do not repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.