Recombinant Human IFNA4 protein(Met1-Asp189), His-tagged

• Cat.No.: IFNA4-175H
• Product Overview: Recombinant Human IFNA4(NP_066546.1)) (Met 1-Asp 189) was expressed in Insect Cells, fused with a polyhistidine tag at the C-terminus.

Specification

• Source: Insect Cells
• Species: Human
• Tag: His
• Protein length: 1-189 a.a.
• Form: Lyophilized from sterile 20mM Tris, 500mM NaCl, pH 7.4, 10% gly. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Bio-activity: Measured in antiviral assays using WISH human amnion cells infected with vesicular stomatitis virus(VSV). The ED50 for this effect is typically 2-8 pg/mL.
• Molecular Mass: The recombinant human IFNα4a consists of 176 amino acids and predicts a molecular mass of 20.7 kDa. It migrates as an approximately 20 kDa band in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 88 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: IFNA4 interferon, alpha 4 [ Homo sapiens ]
• Official Symbol: IFNA4
• Synonyms: IFNA4; interferon, alpha 4; interferon alpha-4; IFN alpha4a; MGC142200; IFN-alpha-4; interferon alpha-4B; interferon alpha-76; interferon alpha-M1; INFA4; IFN-alpha4a
• Gene ID: 3441
• mRNA Refseq: NM_021068
• Protein Refseq: NP_066546
• MIM: 147564
• UniProt ID: P05014