Recombinant Human IDO1, His-tagged

• Cat.No.: IDO1-7189H
• Product Overview: Recombinant Human IDO1 (indoleamine 2,3-dioxygenase) (aa 1-403) fused a His-tag at the C-terminus, was expressed in E. coli.

Specification

• Species: Human
• Source: E.coli
• Tag: Non
• Description: Indoleamine 2,3-dioxygenase(IDO) is a hemecontaining intracellular dioxygenase catalyzing the degradation of the essential amino acid L-tryptophan to N-formylkynurenine. This degradation is the first and ratelimiting step of the L-kynurenine pathway. IDO is widely expressed in dendritic cells, macrophages, microglia, eosinophils, fibroblasts, endothelial cells, and most tumor cells. In immune cells, its expression is mainly induced by cytokines such as IFN-γ, IFN-α, IFN-β, and IL-10. IDO has an antimicrobial function due to its decreasing the availability of the essential amino acid tryptophan in inflammatory environments. Recent studies have demonstrated that IDO induces immunosuppression during infection, pregnancy, transplantation, autoimmunity, and neoplasia.
• Formulation: Liquid. 0.2µm-filtered solution in 50mM TRIS, pH 7.4, containing 1mM EDTA.
• Purity: ≥90% as determined by SDS­PAGE
• EndotoxinContent: <1.0EU/µg protein (LAL test).
• Specific Activity: >100'000U/mg protein with L-tryptophan as substrate. One unit is defined as the amount of enzyme that produces 1nmol of kynurenine per hour.
• Stability: Store at -20°C for long term storage. After opening, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles.
• OfficialSymbol: IDO1

Gene Information

• Gene Name: IDO1 indoleamine 2,3-dioxygenase 1 [ Homo sapiens ]
• Synonyms: IDO1; indoleamine 2,3-dioxygenase 1; IDO; INDO; IDO-1; indoleamine 2,3-dioxygenase 1; EC 1.13.11.52; indole 2,3-dioxygenase; indolamine 2,3 dioxygenase; indoleamine-pyrrole 2,3-dioxygenase; Indoleamine-pyrrole 2
• Gene ID: 3620
• mRNA Refseq: NM_002164
• Protein Refseq: NP_002155
• MIM: 147435
• UniProt ID: P14902
• Chromosome Location: 8p12-p11
• Pathway: African trypanosomiasis; Metabolism of amino acids and derivatives; Tryptophan catabolism; tryptophan degradation XI (mammalian, via kynurenine)
• Function: electron carrier activity; heme binding; indoleamine 2,3-dioxygenase activity; metal ion binding; oxygen binding; tryptophan 2,3-dioxygenase activity; amino acid binding