Recombinant Human ICA1 protein, His-tagged

• Cat.No.: ICA1-25H
• Product Overview: Recombinant Human ICA1(Met1~Gly258) fused with His tag at N-terminal was expressed in E. coli.

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Protein Length: Met1~Gly258
• Description: This gene encodes a protein with an arfaptin homology domain that is found both in the cytosol and as membrane-bound form on the Golgi complex and immature secretory granules. This protein is believed to be an autoantigen in insulin-dependent diabetes mellitus and primary Sjogren's syndrome. Several transcript variants encoding two different isoforms have been found for this gene.
• Form: 20mM Tris, 150mM NaCl,pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.
• Molecular Mass: Predicted Molecular Mass: 36.9kDa;
  Accurate Molecular Mass: 27kDa(Analysis of differences refer to the manual)
• Endotoxin: Less than 1.0 EU per μg by the LAL method.
• Purity: > 95%
• Applications: SDS-PAGE; WB; ELISA; IP; CoIP; Purification; Amine Reactive Labeling
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 centigrade for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 centigrade for one month. Aliquot and store at -80 centigrade for 12 months.
• Reconstitution: Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Gene Information

• Gene Name: ICA1 islet cell autoantigen 1, 69kDa [ Homo sapiens ]
• Official Symbol: ICA1
• Synonyms: ICA1; islet cell autoantigen 1, 69kDa; islet cell autoantigen 1 (69kD); islet cell autoantigen 1; ICAp69; p69; islet cell autoantigen p69; 69 kDa islet cell autoantigen; islet cell autoantigen 1 isoform; diabetes mellitus type I autoantigen; ICA69
• Gene ID: 3382
• mRNA Refseq: NM_001136020
• Protein Refseq: NP_001129492
• MIM: 147625
• UniProt ID: Q05084
• Chromosome Location: 7p22
• Pathway: Type I diabetes mellitus, organism-specific biosystem; Type I diabetes mellitus, conserved biosystem
• Function: molecular_function; protein domain specific binding