Recombinant Human HUS1, His-tagged

• Cat.No.: HUS1-29414TH
• Product Overview: Recombinant full length Human HUS1 with an N terminal His tag; 300 amino acids with tag, Predicted MWt 33.8 kDa.

Specification

• Description: The protein encoded by this gene is a component of an evolutionarily conserved, genotoxin-activated checkpoint complex that is involved in the cell cycle arrest in response to DNA damage. This protein forms a heterotrimeric complex with checkpoint proteins RAD9 and RAD1. In response to DNA damage, the trimeric complex interacts with another protein complex consisting of checkpoint protein RAD17 and four small subunits of the replication factor C (RFC), which loads the combined complex onto the chromatin. The DNA damage induced chromatin binding has been shown to depend on the activation of the checkpoint kinase ATM, and is thought to be an early checkpoint signaling event. Alternative splicing results in multiple transcript variants.
• Protein length: 280 amino acids
• Conjugation: HIS
• Molecular Weight: 33.800kDa inclusive of tags
• Source: E. coli
• Tissue specificity: Ubiquitous.
• Form: Liquid
• Purity: >95% by SDS-PAGE
• Storage buffer: Preservative: NoneConstituents: 40% Glycerol, 20mM Tris HCl, 100mM Sodium chloride, pH 8.0
• Storage: Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
• Sequences of amino acids: MGSSHHHHHHSSGLVPRGSHMKFRAKIVDGACLNHFTRISNMIAKLAKTCTLRISPDKLNFILCDKLANGGVSMWCELEQENFFNEFQMEGVSAENNEIYLELTSENLSRALKTAQNARALKIKLTNKHFPCLTVSVELLSMSSSSRIVTHDIPIKVIPRKLWKDLQEPVVPDPDVSIYLPVLKTMKSVVEKMKNISNHLVIEANLDGELNLKIETELVCVTTHFKDLGNPPLASESTHEDRNVEHMAEVHIDIRKLLQFLAGQQVNPTKALCNIVNNKMVHFDLLHEDVSLQYFIPALS
• Sequence Similarities: Belongs to the HUS1 family.

Gene Information

• Gene Name: HUS1 HUS1 checkpoint homolog (S. pombe) [ Homo sapiens ]
• Official Symbol: HUS1
• Synonyms: HUS1; HUS1 checkpoint homolog (S. pombe); HUS1 (S. pombe) checkpoint homolog; checkpoint protein HUS1; hus1+ like protein
• Gene ID: 3364
• mRNA Refseq: NM_004507
• Protein Refseq: NP_004498
• MIM: 603760
• Uniprot ID: O60921
• Chromosome Location: 7p13-p12
• Pathway: Activation of ATR in response to replication stress, organism-specific biosystem; Cell Cycle Checkpoints, organism-specific biosystem; G2/M Checkpoints, organism-specific biosystem; Regulation of Telomerase, organism-specific biosystem
• Function: protein binding; protein serine/threonine kinase activity