Recombinant Human HTRA4 Protein, His-tagged

• Cat.No.: HTRA4-2262H
• Product Overview: Recombinant Human HTRA4 Protein (Gly202-Leu362) with a N-His tag was expressed in E. coli.

Specification

• Description: This gene encodes a member of the HtrA family of proteases. The encoded protein contains a putative signal peptide, an insulin growth factor binding domain, a Kazal protease inhibitor domain, a conserved trypsin domain and a PDZ domain. Based on studies on other related family members, this enzyme may function as a secreted oligomeric chaperone protease to degrade misfolded secretory proteins. Other human HtrA proteins have been implicated in arthritis, tumor suppression, unfolded stress response, apoptosis, and aging.
• Source: E. coli
• Species: Human
• Tag: His
• Form: Freeze-dried powder
• Molecular Mass: Predicted Molecular Mass: 20.9 kDa
  Accurate Molecular Mass: 21 kDa
• Protein length: Gly202-Leu362
• Endotoxin: <1.0 EU per 1µg (determined by the LAL method).
• Purity: > 90%
• Applications: Positive Control; Immunogen; SDS-PAGE; WB.
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 centigrade for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 centigrade for one month. Aliquot and store at -80 centigrade for 12 months.
• Storage Buffer: 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.
• Reconstitution: Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Gene Information

• Gene Name: HTRA4 HtrA serine peptidase 4 [ Homo sapiens (human) ]
• Official Symbol: HTRA4
• Synonyms: HTRA4; HtrA serine peptidase 4; probable serine protease HTRA4; FLJ90724
• Gene ID: 203100
• mRNA Refseq: NM_153692
• Protein Refseq: NP_710159
• MIM: 610700
• UniProt ID: P83105