Recombinant Human HTRA1 Protein, MYC/DDK-tagged, C13 and N15-labeled

• Cat.No.: HTRA1-090H
• Product Overview: HTRA1 MS Standard C13 and N15-labeled recombinant protein (NP_002766) with a C-terminal MYC/DDK tag, was expressed in HEK293 cells.

Specification

• Description: This gene encodes a member of the trypsin family of serine proteases. This protein is a secreted enzyme that is proposed to regulate the availability of insulin-like growth factors (IGFs) by cleaving IGF-binding proteins. It has also been suggested to be a regulator of cell growth. Variations in the promoter region of this gene are the cause of susceptibility to age-related macular degeneration type 7.
• Source: HEK293
• Species: Human
• Tag: Myc&DDK
• Molecular Mass: 51.53 kDa
• AA Sequence: MQIPRAALLPLLLLLLAAPASAQLS RAGRSAPLAAGCPDRCEPARCPPQP EHCEGGRARDACGCCEVCGAPEGAA CGLQEGPCGEGLQCVVPFGVPASAT VRRRAQAGLCVCASSEPVCGSDANT YANLCQLRAASRRSERLHRPPVIVL QRGACGQGQEDPNSLRHKYNFIADV VEKIAPAVVHIELFRKLPFSKREVP VASGSGFIVSEDGLIVTNAHVVTNK HRVKVELKNGATYEAKIKDVDEKAD IALIKIDHQGKLPVLLLGRSSELRP GEFVVAIGSPFSLQNTVTTGIVSTT QRGGKELGLRNSDMDYIQTDAIINY GNSGGPLVNLDGEVIGINTLKVTAG ISFAIPSDKIKKFLTESHDRQAKGK AITKKKYIGIRMMSLTSSKAKELKD RHRDFPDVISGAYIIEVIPDTPAEA GGLKENDVIISINGQSVVSANDVSD VIKRESTLNMVVRRGNEDIMITVIP EEIDPSGPSGPTRTRRLEQKLISEE DLAANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 3 months from receipt of products under proper storage and handling conditions.
• Storage: Store at -80 centigrade. Avoid repeated freeze-thaw cycles.
• Concentration: 50 μg/mL as determined by BCA
• Storage Buffer: 100 mM glycine, 25 mM Tris-HCl, pH 7.3.

Gene Information

• Gene Name: HTRA1 HtrA serine peptidase 1 [ Homo sapiens (human) ]
• Official Symbol: HTRA1
• Synonyms: HTRA1; HtrA serine peptidase 1; ARMD7; CADASIL2; CARASIL; HtrA; L56; ORF480; PRSS11; serine protease HTRA1; IGFBP5-protease; high-temperature requirement A serine peptidase 1; protease, serine, 11 (IGF binding); EC 3.4.21.-
• Gene ID: 5654
• mRNA Refseq: NM_002775
• Protein Refseq: NP_002766
• MIM: 602194
• UniProt ID: Q92743

Reviews

06/06/2022

The structure of HTRA1 is stable and not easy to mutate, which ensures the reliability of the experimental results.

08/17/2021

The solubility and ion exchange capacity are very good, which is convenient for subsequent experimental processing and purification.

11/10/2020

It is easy to operate and does not require complex operation steps.

Q&As

Is there a relationship between the structure and function of HTRA1 protein?

The structure of the HTRA1 protein determines its function. The serine protease domain is the main functional region of HTRA1 protein, which has catalytic activity and can degrade proteins. In addition, the HTRA1 protein has a number of different domains that are closely related to its function.

Describe the treatment strategy of HTRA1 protein?

Therapeutic strategies for HTRA1 protein include inhibiting its activity, regulating its expression level, and altering its structure. Several drugs are already undergoing clinical trials to evaluate their efficacy against cancer and other diseases.

How can disease be treated by regulating the expression of HTRA1 protein?

There are currently no treatments that directly regulate HTRA1 protein expression. However, some diseases can be treated by inhibiting their activity or regulating their expression levels. For example, some drugs can inhibit the activity of the HTRA1 protein to treat cancer or Alzheimer's disease.

Is HTRA1 involved in cell signal transduction?

HTRA1 protein can be used as a signal transduction molecule by binding to receptors on the cell surface to transmit signals to the cell interior and participate in cell proliferation, differentiation and apoptosis.

How does HTRA1 protein participate in extracellular matrix remodeling?

This protein can degrade a variety of proteins in the extracellular matrix, including collagen, elastin and fibrin, and thus participate in extracellular matrix remodeling.

What method is used to detect the expression level of HTRA1 protein in biological samples?

The expression level of HTRA1 protein in biological samples can be detected by immunohistochemistry, western blot and real-time quantitative PCR.