Recombinant Human HTR3A 293 Cell Lysate

• Cat.No.: HTR3A-5334HCL

Specification

• Species: Human
• Source: HEK293
• Tag: Non
• Description: Antigen standard for 5-hydroxytryptamine (serotonin) receptor 3A (HTR3A), transcript variant 1 is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
• Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
• Size: 0.1 mg
• Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
• Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.

Gene Information

• Gene Name: HTR3A 5-hydroxytryptamine (serotonin) receptor 3A, ionotropic [ Homo sapiens ]
• Official Symbol: HTR3A
• Synonyms: HTR3A; 5-hydroxytryptamine (serotonin) receptor 3A, ionotropic; 5 hydroxytryptamine (serotonin) receptor 3A , HTR3; 5-hydroxytryptamine receptor 3A; 5 HT3A; 5 HT3R; 5-HT3-A; serotonin receptor 3A; 5HT3 serotonin receptor; 5-hydroxytryptamine receptor 3; serotonin-gated ion channel receptor; HTR3; 5HT3R; 5-HT-3; 5-HT3A; 5-HT3R
• Gene ID: 3359
• mRNA Refseq: NM_000869
• Protein Refseq: NP_000860
• MIM: 182139
• UniProt ID: P46098
• Chromosome Location: 11q23.1-q23.2
• Pathway: Ion channel transport, organism-specific biosystem; Ligand-gated ion channel transport, organism-specific biosystem; Serotonergic synapse, organism-specific biosystem; Transmembrane transport of small molecules, organism-specific biosystem
• Function: extracellular ligand-gated ion channel activity; extracellular ligand-gated ion channel activity; ion channel activity; receptor activity; serotonin binding; serotonin receptor activity; serotonin receptor activity; serotonin-activated cation-selective channel activity