Recombinant Human HSPD1 protein(Leu2-Phe573), His&GST-tagged

• Cat.No.: HSPD1-2373H
• Product Overview: Recombinant Human HSP60 (NP_955472.1) (Leu 2-Phe 573) was expressed in E. coli, fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.

Specification

• Species: Human
• Source: E.coli
• Tag: GST&His
• Protein Length: Leu2-Phe573
• Form: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Molecular Mass: The recombinant human HSP60/GST chimera consists of 809 amino acids and has a calculated molecular mass of 88.7 KDa. It migrates as an approximately 52-65 kDa band in SDS-PAGE under reducing conditions.
• Purity: > 90 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: HSPD1 heat shock 60kDa protein 1 (chaperonin) [ Homo sapiens ]
• Official Symbol: HSPD1
• Synonyms: HSPD1; heat shock 60kDa protein 1 (chaperonin); heat shock 60kD protein 1 (chaperonin) , spastic paraplegia 13 (autosomal dominant) , SPG13; 60 kDa heat shock protein, mitochondrial; GROEL; HSP60; chaperonin 60; 60 kDa chaperonin; heat shock protein 65; P60 lymphocyte protein; mitochondrial matrix protein P1; short heat shock protein 60 Hsp60s1; HLD4; CPN60; HSP65; SPG13; HSP-60; HuCHA60
• Gene ID: 3329
• mRNA Refseq: NM_002156
• Protein Refseq: NP_002147
• MIM: 118190
• UniProt ID: P10809