Recombinant Human HSPB3 protein, His-tagged

• Cat.No.: HSPB3-2729H
• Product Overview: Recombinant Human HSPB3 protein(1-130 aa), fused to His tag, was expressed in E. coli.

Specification

• Source: E. coli
• Species: Human
• Tag: His
• Form: The purified protein was Lyophilized from sterile PBS (58mM Na2HPO4,17mM NaH2PO4, 68mM NaCl, pH7.4). 5 % trehalose and 5 % mannitol are added as protectant before lyophilization. The elution buffer contain 300mM imidazole.
• Protein length: 1-130 aa
• AA Sequence: MAKIILRHLIEIPVRYQEEFEARGLEDCRLDHALYALPGPTIVDLRKTRAAQSPPVDSAAETPPREGKSHFQILLDVVQFLPEDIIIQTFEGWLLIKAQHGTRMDEHGFISRSFTRQYKLPDGVEIKDLS
• Purity: 90%, by SDS-PAGE with Coomassie Brilliant Blue staining.
• Storage: Short-term storage: Store at 2-8°C for (1-2 weeks).
  Long-term storage: Aliquot and store at -20°C to -80°C for up to 3 months, buffer containing 50% glycerol is recommended for reconstitution. Avoid repeat freeze-thaw cycles.
• Reconstitution: Reconstitute at 0.25 µg/μl in 200 μl sterile water for short-term storage.
  Reconstitution with 200 μl 50% glycerol solution is recommended for longer term storage.

Gene Information

• Gene Name: HSPB3 heat shock 27kDa protein 3 [ Homo sapiens ]
• Official Symbol: HSPB3
• Synonyms: HSPB3; heat shock 27kDa protein 3; heat shock 27kD protein 3; heat shock protein beta-3; HSPL27; HSP 17; protein 3; heat shock 17 kDa protein; HMN2C; DHMN2C
• Gene ID: 8988
• mRNA Refseq: NM_006308
• Protein Refseq: NP_006299
• MIM: 604624
• UniProt ID: Q12988