Recombinant Human HSPA9 293 Cell Lysate

• Cat.No.: HSPA9-5352HCL

Specification

• Species: Human
• Source: HEK293
• Tag: Non
• Description: Antigen standard for heat shock 70kDa protein 9 (mortalin) (HSPA9), nuclear gene encoding mitochondrial protein is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
• Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
• Size: 0.1 mg
• Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
• Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.

Gene Information

• Gene Name: HSPA9 heat shock 70kDa protein 9 (mortalin) [ Homo sapiens ]
• Official Symbol: HSPA9
• Synonyms: HSPA9; heat shock 70kDa protein 9 (mortalin); heat shock 70kDa protein 9B (mortalin 2) , HSPA9B; stress-70 protein, mitochondrial; GRP75; mot 2; mthsp75; PBP74; mortalin-2; p66-mortalin; mortalin, perinuclear; heat shock 70kD protein 9B; peptide-binding protein 74; 75 kDa glucose-regulated protein; CSA; MOT; MOT2; GRP-75; HSPA9B; MTHSP75; MGC4500
• Gene ID: 3313
• mRNA Refseq: NM_004134
• Protein Refseq: NP_004125
• MIM: 600548
• UniProt ID: P38646
• Chromosome Location: 5q31.1
• Pathway: Metabolism of proteins, organism-specific biosystem; Mitochondrial Protein Import, organism-specific biosystem; RNA degradation, organism-specific biosystem; RNA degradation, conserved biosystem; Tuberculosis, organism-specific biosystem; Tuberculosis, conserved biosystem
• Function: ATP binding; enzyme binding; nucleotide binding; protein binding; unfolded protein binding