Recombinant Human HSD17B12 protein, His-tagged

• Cat.No.: HSD17B12-1409H
• Product Overview: Recombinant Human HSD17B12 aa. (Met1~Asn312 fused with N-terminal His tag was produced in E. coli cells.

Specification

• Description: This gene encodes a very important 17beta-hydroxysteroid dehydrogenase (17beta-HSD) that converts estrone into estradiol in ovarian tissue. This enzyme is also involved in fatty acid elongation.
• Source: E. coli
• Species: Human
• Tag: His
• Form: Freeze-dried powder
• Molecular Mass: 38kDa as determined by SDS-PAGE reducing conditions.
• Protein length: Met1~Asn312
• Endotoxin: <1.0EU per 1µg (determined by the LAL method)
• Purity: >98%
• Characteristic: The isoelectric point is 9.3.
• Applications: SDS-PAGE; WB; ELISA; IP; CoIP; Purification; Amine Reactive Labeling.
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
• Concentration: 200μg/mL
• Storage buffer: 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% sarcosyl, 5% Trehalose and Proclin300.
• Reconstitution: Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0mg/mL. Do not vortex.

Gene Information

• Gene Name: HSD17B12 hydroxysteroid 17-beta dehydrogenase 12 [ Homo sapiens (human) ]
• Official Symbol: HSD17B12
• Synonyms: HSD17B12; hydroxysteroid 17-beta dehydrogenase 12; KAR; SDR12C1; very-long-chain 3-oxoacyl-CoA reductase; 17-beta-HSD 12; 17-beta-hydroxysteroid dehydrogenase 12; 17beta-HSD type 12; 3-ketoacyl-CoA reductase; estradiol 17-beta-dehydrogenase 12; short chain dehydrogenase/reductase family 12C member 1; steroid dehydrogenase homolog
• Gene ID: 51144
• mRNA Refseq: NM_016142.2
• Protein Refseq: NP_057226.1
• UniProt ID: Q53GQ0