Recombinant Human HSD11B2, GST-tagged

• Cat.No.: HSD11B2-13953H
• Product Overview: Recombinant Human HSD11B2 (1 a.a. - 405 a.a.), fused with GST-tag at N-terminal, was expressed in wheat germ.

Specification

• Description: There are at least two isozymes of the corticosteroid 11-beta-dehydrogenase, a microsomal enzyme complex responsible for the interconversion of cortisol and cortisone. The type I isozyme has both 11-beta-dehydrogenase (cortisol to cortisone) and 11-oxoreductase (cortisone to cortisol) activities. The type II isozyme, encoded by this gene, has only 11-beta-dehydrogenase activity. In aldosterone-selective epithelial tissues such as the kidney, the type II isozyme catalyzes the glucocorticoid cortisol to the inactive metabolite cortisone, thus preventing illicit activation of the mineralocorticoid receptor. In tissues that do not express the mineralocorticoid receptor, such as the placenta and testis, it protects cells from the growth-inhibiting and/or pro-apoptotic effects of cortisol, particularly during embryonic development. Mutations in this gene cause the syndrome of apparent mineralocorticoid excess and hypertension.
• Source: Wheat germ
• Species: Human
• Tag: GST
• Molecular Mass: 70.5 kDa
• AA Sequence: MERWPWPSGGAWLLVAARALLQLLRSDLRLGRPLLAALALLAALDWLCQRLLPPPAALAVLAAAGWIALSRLARP QRLPVATRAVLITGCDSGFGKETAKKLDSMGFTVLATVLELNSPGAIELRTCCSPRLRLLQMDLTKPGDISRVLE FTKAHTTSTGLWGLVNNAGHNEVVADAELSPVATFRSCMEVNFFGALELTKGLLPLLRSSRGRIVTVGSPAGDMP YPCLGAYGTSKAAVALLMDTFSCELLPWGVKVSIIQPGCFKTESVRNVGQWEKRKQLLLANLPQELLQAYGKDYI EHLHGQFLHSLRLAMSDLTPVVDAITDALLAARPRRRYYPGQGLGLMYFTHYYLPEGLRRRFLQAFFISHCLPRA LQPGQPGTTPPQDAAQDPNLSPGPSPAVAR
• Applications: ELISA; WB-Re; AP; Array
• Notes: Best use within three months from the date of receipt of this protein.
• Storage: Store at -80°C. Aliquot to avoid repeated freezing and thawing.
• Storage Buffer: 50 mM Tris-HCI, 10 mM reduced Glutathione, pH=8.0 in the elution buffer.

Gene Information

• Gene Name: HSD11B2 hydroxysteroid (11-beta) dehydrogenase 2 [ Homo sapiens (human) ]
• Official Symbol: HSD11B2
• Synonyms: HSD11B2; AME; AME1; HSD2; HSD11K; SDR9C3; hydroxysteroid (11-beta) dehydrogenase 2; corticosteroid 11-beta-dehydrogenase isozyme 2; 11-DH2; 11-beta-HSD2; -HSD11 type II; 11-beta-hydroxysteroid dehydrogenase type 2; 11-beta-hydroxysteroid dehydrogenase type II; NAD-dependent 11-beta-hydroxysteroid dehydrogenase; short chain dehydrogenase/reductase family 9C member 3; NP_000187.3; EC 1.1.1.146
• Gene ID: 3291
• mRNA Refseq: NM_000196
• Protein Refseq: NP_000187
• MIM: 614232
• UniProt ID: P80365
• Chromosome Location: 16q22
• Pathway: Aldosterone-regulated sodium reabsorption; C21-Steroid hormone biosynthesis, progesterone => cortisol/cortisone; Glucocorticoid & Mineralcorticoid Metabolism
• Function: 11-beta-hydroxysteroid dehydrogenase [NAD(P)] activity; NAD binding; steroid binding