Recombinant Human HS3ST5 293 Cell Lysate

• Cat.No.: HS3ST5-5385HCL

Specification

• Description: Antigen standard for heparan sulfate (glucosamine) 3-O-sulfotransferase 5 (HS3ST5) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid (True ORF cDNA clone RC210876, OriGene Technologies, Inc.) and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
• Source: HEK 293 cells
• Species: Human
• Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
• Size: 0.1 mg
• Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
• Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
• Tag: Non

Gene Information

• Gene Name: HS3ST5 heparan sulfate (glucosamine) 3-O-sulfotransferase 5 [ Homo sapiens ]
• Official Symbol: HS3ST5
• Synonyms: HS3ST5; heparan sulfate (glucosamine) 3-O-sulfotransferase 5; heparan sulfate glucosamine 3-O-sulfotransferase 5; 3 OST 5; h3-OST-5; heparan sulfate 3-OST-5; heparan sulfate 3-O-sulfotransferase 5; heparan sulfate D-glucosaminyl 3-O-sulfotransferase 5; 3OST5; 3-OST-5; HS3OST5; NBLA04021
• Gene ID: 222537
• mRNA Refseq: NM_153612
• Protein Refseq: NP_705840
• MIM: 609407
• UniProt ID: Q8IZT8
• Chromosome Location: 6q21
• Pathway: Glycosaminoglycan biosynthesis - heparan sulfate, organism-specific biosystem; Glycosaminoglycan biosynthesis - heparan sulfate, conserved biosystem
• Function: 3-phosphoadenosine 5-phosphosulfate binding; [heparan sulfate]-glucosamine 3-sulfotransferase 1 activity; protein binding; sulfotransferase activity; transferase activity