Recombinant Human HS2ST1 protein, His & T7-tagged

• Cat.No.: HS2ST1-1591H
• Product Overview: Recombinant Human HS2ST1 aa. (Asn29~Asn356 (Accession # Q7LGA3)) fused with N-terminal His & T7 tag was produced in E. coli cells.

Specification

• Species: Human
• Source: E.coli
• Tag: His&T7
• Protein Length: Asn29~Asn356
• Description: Heparan sulfate biosynthetic enzymes are key components in generating a myriad of distinct heparan sulfate fine structures that carry out multiple biologic activities. This gene encodes a member of the heparan sulfate biosynthetic enzyme family that transfers sulfate to the 2 position of the iduronic acid residue of heparan sulfate. The disruption of this gene resulted in no kidney formation in knockout embryonic mice, indicating that the absence of this enzyme may interfere with the signaling required for kidney formation. Two alternatively spliced transcript variants that encode different proteins have been found for this gene.
• Form: Freeze-dried powder
• Molecular Mass: Predicted Molecular Mass: 42.5kDa
• Endotoxin: <1.0EU per 1ug (determined by the LAL method)
• Purity: >90%
• Applications: SDS-PAGE; WB; ELISA; IP.
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
• Storage buffer: Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl.
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Isoelectric Point: 8.7

Gene Information

• Gene Name: HS2ST1 heparan sulfate 2-O-sulfotransferase 1 [ Homo sapiens (human) ]
• Official Symbol: HS2ST1
• Synonyms: HS2ST1; heparan sulfate 2-O-sulfotransferase 1; dJ604K5.2; 2-O-sulfotransferase; 2OST
• Gene ID: 9653
• mRNA Refseq: NM_001134492.1
• Protein Refseq: NP_001127964.1
• UniProt ID: Q7LGA3