Recombinant Human HRG protein(Val19~Lys525), His-tagged

• Cat.No.: HRG-593H
• Product Overview: Recombinant Human HRG protein(Val19~Lys525), fused with N-terminal His tag, was expressed in HEK293.

Specification

• Species: Human
• Source: HEK293
• Tag: His
• Protein Length: Val19~Lys525
• Form: 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% sarcosyl, 5% trehalose, and Proclin300.
• Bio-activity: HRG (Histidine-rich glycoprotein) is a plasma glycoprotein which is distinguished by its high content of histidine and proline. HRG binds a number of ligands such as heme, heparin, heparan sulfate, thrombospondin, plasminogen, and divalent metal ions. Thus, a functional binding ELISA assay was constructed to detect the association of rhHRG with heparin. Briefly, Microtiter wells were coated with OVA-conjugated-heparin. rhHRG were diluted serially in 0.01M PBS (pH 7.4). Duplicate samples of 100uL were then transferred to the coated microtiter wells and incubated for 2h at 37°C. Wells were washed with PBST and incubated for 1h with anti-HRG PAb, then aspirated and washed 3 times. After incubation with HRP labeled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate Solution, wells were incubated 15-25 minutes at 37°C. Finally, add 50μL stop solution to the wells and read at 450nm immediately. The binding activity of rhHRG with heparin was shown in Figure and this effect was in a dose dependent manner.
• Molecular Mass: 59.3kDa
• Endotoxin: <1.0EU per 1μg (determined by the LAL method).
• Purity: >98%
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
• Reconstitution: Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Gene Information

• Gene Name: HRG histidine-rich glycoprotein [ Homo sapiens ]
• Official Symbol: HRG
• Synonyms: HRG; histidine-rich glycoprotein; histidine proline rich glycoprotein; HPRG; HRGP; thrombophilia due to elevated HRG; histidine-proline-rich glycoprotein; THPH11; DKFZp779H1622
• Gene ID: 3273
• mRNA Refseq: NM_000412
• Protein Refseq: NP_000403
• MIM: 142640
• UniProt ID: P04196