Recombinant Human HRG protein, His-tagged

• Cat.No.: HRG-514H
• Product Overview: Recombinant Human HRG (P04196) aa. (Val19~Lys525) fused with His tag was produced in HEK293F cells.

Specification

• Species: Human
• Source: HEK293
• Tag: His
• Protein Length: Val19~Lys525
• Description: This histidine-rich glycoprotein contains two cystatin-like domains and is located in plasma and platelets. The physiological function has not been determined but it is known that the protein binds heme, dyes and divalent metal ions. The encoded protein also has a peptide that displays antimicrobial activity against C. albicans, E. coli, S. aureus, P. aeruginosa, and E. faecalis. It can inhibit rosette formation and interacts with heparin, thrombospondin and plasminogen. Two of the protein's effects, the inhibition of fibrinolysis and the reduction of inhibition of coagulation, indicate a potential prothrombotic effect. Mutations in this gene lead to thrombophilia due to abnormal histidine-rich glycoprotein levels.
• Form: Freeze-dried powder
• Molecular Mass: Predicted Molecular Mass: 59.3kDa; Accurate Molecular Mass: 80kDa as determined by SDS-PAGE reducing conditions.
• Endotoxin: <1.0EU per 1ug (determined by the LAL method)
• Purity: > 97%
• Characteristic: Isoelectric point is 7.
• Applications: SDS-PAGE; WB; ELISA; IP; CoIP; EMSA; Reporter Assays; Purification; Amine Reactive Labeling
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
• Concentration: 200ug/mL
• Storage buffer: 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 5% Trehalose and Proclin300.
• Reconstitution: Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex

Gene Information

• Gene Name: HRG histidine rich glycoprotein [ Homo sapiens (human) ]
• Official Symbol: HRG
• Synonyms: HRG; histidine rich glycoprotein; HPRG; HRGP; THPH11; histidine-rich glycoprotein;
  histidine-proline-rich glycoprotein
• Gene ID: 3273
• mRNA Refseq: NM_000412.4
• Protein Refseq: NP_000403.1
• UniProt ID: P04196