Recombinant Human HP, His-tagged

• Cat.No.: HP-5101H
• Product Overview: Recombinant human HP protein, fused to His-tag at N-terminus, was expressed in E.coli.

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Description: HP, as known as haptoglobin, is processed to yield both alpha and beta chains, which subsequently combine as a tetramer to produce haptoglobin. In blood plasma, haptoglobin binds free hemoglobin (Hb) released from erythrocytes with high affinity and thereby inhibits its oxidative activity. The haptoglobin-hemoglobin complex will then be removed by the reticuloendothelial system (mostly the spleen).
• Form: Liquid. 20mM Tris-HCl buffer (pH8.0) containing 10% glycerol 0.4M Urea
• Molecular Mass: 39.0 kDa(352aa)
• AA Sequence: MGSSHHHHHH SSGLVPRGSH MGSVDSGNDV TDIADDGCPK PPEIAHGYVE HSVRYQCKNY YKLRTEGDGV YTLNNEKQWI NKAVGDKLPE CEAVCGKPKN PANPVQRILG GHLDAKGSFP WQAKMVSHHN LTTGATLINE QWLLTTAKNL FLNHSENATA KDIAPTLTLY VGKKQLVEIE KVVLHPNYSQ VDIGLIKLKQ KVSVNERVMP ICLPSKDYAE VGRVGYVSGW GRNANFKFTD HLKYVMLPVA DQDQCIRHYE GSTVPEKKTP KSPVGVQPIL NEHTFCAGMS KYQEDTCYGD AGSAFAVHDL EEDTWYATGI LSFDKSCAVA EYGVYVKVTS IQDWVQKTIA EN
• Purity: >80% by SDS - PAGE
• Applications: SDS-PAGE
• Storage: Can be stored at +4centigrade short term (1-2 weeks). For long term storage, aliquot and store at -20centigrade or -70centigrade. Avoid repeated freezing and thawing cycles.
• Concentration: 0.5mg/ml (determined by Bradford assay)

Gene Information

• Gene Name: HP haptoglobin [ Homo sapiens ]
• Official Symbol: HP
• Synonyms: HP; haptoglobin; zonulin; binding peptide; haptoglobin alpha(1S)-beta; haptoglobin alpha(2FS)-beta; haptoglobin, beta polypeptide; haptoglobin, alpha polypeptide; BP; HPA1S; HP2ALPHA2; MGC111141
• Gene ID: 3240
• mRNA Refseq: NM_001126102
• Protein Refseq: NP_001119574
• MIM: 140100
• UniProt ID: P00738
• Chromosome Location: 16q22.2
• Pathway: amb2 Integrin signaling, organism-specific biosystem
• Function: catalytic activity; hemoglobin binding; serine-type endopeptidase activity