Recombinant Human HP, His-tagged

• Cat.No.: HP-5101H
• Product Overview: Recombinant human HP protein, fused to His-tag at N-terminus, was expressed in E.coli.

Specification

• Description: HP, as known as haptoglobin, is processed to yield both alpha and beta chains, which subsequently combine as a tetramer to produce haptoglobin. In blood plasma, haptoglobin binds free hemoglobin (Hb) released from erythrocytes with high affinity and thereby inhibits its oxidative activity. The haptoglobin-hemoglobin complex will then be removed by the reticuloendothelial system (mostly the spleen).
• Source: E. coli
• Species: Human
• Tag: His
• Form: Liquid. 20mM Tris-HCl buffer (pH8.0) containing 10% glycerol 0.4M Urea
• Molecular Mass: 39.0 kDa(352aa)
• AA Sequence: MGSSHHHHHH SSGLVPRGSH MGSVDSGNDV TDIADDGCPK PPEIAHGYVE HSVRYQCKNY YKLRTEGDGV YTLNNEKQWI NKAVGDKLPE CEAVCGKPKN PANPVQRILG GHLDAKGSFP WQAKMVSHHN LTTGATLINE QWLLTTAKNL FLNHSENATA KDIAPTLTLY VGKKQLVEIE KVVLHPNYSQ VDIGLIKLKQ KVSVNERVMP ICLPSKDYAE VGRVGYVSGW GRNANFKFTD HLKYVMLPVA DQDQCIRHYE GSTVPEKKTP KSPVGVQPIL NEHTFCAGMS KYQEDTCYGD AGSAFAVHDL EEDTWYATGI LSFDKSCAVA EYGVYVKVTS IQDWVQKTIA EN
• Purity: >80% by SDS - PAGE
• Applications: SDS-PAGE
• Storage: Can be stored at +4centigrade short term (1-2 weeks). For long term storage, aliquot and store at -20centigrade or -70centigrade. Avoid repeated freezing and thawing cycles.
• Concentration: 0.5mg/ml (determined by Bradford assay)

Gene Information

• Gene Name: HP haptoglobin [ Homo sapiens ]
• Official Symbol: HP
• Synonyms: HP; haptoglobin; zonulin; binding peptide; haptoglobin alpha(1S)-beta; haptoglobin alpha(2FS)-beta; haptoglobin, beta polypeptide; haptoglobin, alpha polypeptide; BP; HPA1S; HP2ALPHA2; MGC111141
• Gene ID: 3240
• mRNA Refseq: NM_001126102
• Protein Refseq: NP_001119574
• MIM: 140100
• UniProt ID: P00738
• Chromosome Location: 16q22.2
• Pathway: amb2 Integrin signaling, organism-specific biosystem
• Function: catalytic activity; hemoglobin binding; serine-type endopeptidase activity

Reviews

07/02/2022

Its superior purity and stability ensure reliable and reproducible results, providing me with confidence in the integrity of my experiments.

01/18/2020

Its impeccable purity and stability ensure accurate and reproducible results, giving me confidence in the reliability of my research outcomes

07/07/2019

Whether I am investigating protein-protein interactions, studying enzymatic activity, or conducting protein structure analyses, the HP protein consistently delivers exceptional performance and accurate results.

Q&As

Can Haptoglobin levels be influenced by genetic factors?

Yes, genetic variations can affect Haptoglobin levels, and understanding these variations can be crucial for personalized medicine approaches.

How does Haptoglobin contribute to the body's defense against oxidative stress?

Haptoglobin acts as an antioxidant by binding to free hemoglobin, preventing the formation of reactive oxygen species that contribute to oxidative stress.

Are there any specific diseases where monitoring Haptoglobin is particularly crucial?

Conditions such as hemolytic anemias, infections, and chronic inflammatory diseases often require monitoring Haptoglobin levels for diagnostic and prognostic purposes.

How is the Haptoglobin test performed in a clinical setting?

The Haptoglobin test is usually conducted through a blood sample, and the serum or plasma is analyzed for Haptoglobin levels.

Can Haptoglobin be a diagnostic marker for conditions other than hemolysis and inflammation?

Yes, altered levels of Haptoglobin are associated with various conditions, including cardiovascular diseases, infections, and certain cancers.