Recombinant Human HLA-E protein, His-tagged

• Cat.No.: HLA-E-1746H
• Product Overview: Recombinant Human HLA-E aa. (Gly22~Leu358) fused with N-terminal His tag was produced in E. coli cells.

Specification

• Description: HLA-E belongs to the HLA class I heavy chain paralogues. This class I molecule is a heterodimer consisting of a heavy chain and a light chain (beta-2 microglobulin). The heavy chain is anchored in the membrane. HLA-E binds a restricted subset of peptides derived from the leader peptides of other class I molecules. The heavy chain is approximately 45 kDa and its gene contains 8 exons. Exon one encodes the leader peptide, exons 2 and 3 encode the alpha1 and alpha2 domains, which both bind the peptide, exon 4 encodes the alpha3 domain, exon 5 encodes the transmembrane region, and exons 6 and 7 encode the cytoplasmic tail.
• Source: E. coli
• Species: Human
• Tag: His
• Form: Freeze-dried powder
• Molecular Mass: 42kDa as determined by SDS-PAGE reducing conditions.
• Protein length: Gly22~Leu358
• Endotoxin: <1.0EU per 1ug (determined by the LAL method)
• Purity: >95%
• Characteristic: The isoelectric point is 5.8.
• Applications: Positive Control; Immunogen; SDS-PAGE; WB.
• Stability: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
• Concentration: 200μg/mL
• Storage Buffer: 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% sarcosyl, 5% Trehalose and Proclin300.
• Reconstitution: Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0mg/mL. Do not vortex.

Gene Information

• Gene Name: HLA-E major histocompatibility complex, class I, E [ Homo sapiens (human) ]
• Official Symbol: HLA-E
• Synonyms: CA12; carbonic anhydrase 12; CAXII; CA-XII; T18816; HsT18816; carbonate dehydratase XII; carbonic anhydrase XII; carbonic dehydratase; tumor antigen HOM-RCC-3.1.3
• Gene ID: 3133
• mRNA Refseq: NM_005516.5
• Protein Refseq: NP_005507.3
• UniProt ID: P13747