Recombinant Human HLA-E*01:03&B2M&Peptide (VMAPRTLVL) Tetramer protein, His-Avi-tagged, Biotinylated

• Cat.No.: HLA-E&B2M-4643H
• Product Overview: Recombinant Biotinylated Human HLA-E*01:03 Tetramer Protein(Gly25-Ile305(HLA-E*01:03), Ile21-Met119(B2M) and VMAPRTLVL peptide) is expressed from HEK293 with His tag and Avi tag at the C-terminal, tetramer is assembled by Biotinylated monomer and streptavidin.

Specification

• Species: Human
• Source: HEK293
• Tag: His&Avi
• ProteinLength: Gly25-Ile305(HLA-E*01:03), Ile21-Met119(B2M)and VMAPRTLVL peptide
• Tag: C-His-Avi
• Conjugation/Label: Biotin
• Form: Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
• Bio-activity: Immobilized Biotinylated Human HLA-E*01:03&B2M&Peptide (VMAPRTLVL) Tetramer, His Tag at 0.5μg/ml (100μl/Well) on streptavidin (5μg/ml) precoated plate. Dose response curve for Anti-B2M Antibody, mFc Tag with the EC50 of 7.7ng/ml determined by ELISA.
• Molecular Mass: The protein has a predicted MW of 258 kDa. Due to glycosylation, the protein migrates to 260-265 kDa under Non reducing (N) condition based on Tris-Bis PAGE result.
• Endotoxin: Less than 1EU per μg by the LAL method.
• Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
• Storage: Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
• Reconstitution: Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
• GeneID 2: 567

Gene Information

• Gene Name: HLA-E major histocompatibility complex, class I, E [ Homo sapiens ]
• Official Symbol: HLA-E
• Synonyms: HLA-E; major histocompatibility complex, class I, E; HLA class I histocompatibility antigen, alpha chain E; MHC HLA-E alpha-1; lymphocyte antigen; MHC HLA-E alpha-2.1; MHC class I antigen E; HLA class I histocompatibility antigen, E alpha chain; MHC; QA1; EA1.2; EA2.1; HLA-6.2; DKFZp686P19218
• Gene ID: 3133
• mRNA Refseq: NM_005516
• Protein Refseq: NP_005507
• MIM: 143010
• UniProt ID: P13747
• Gene Name 2: B2M beta-2-microglobulin [ Homo sapiens ]
• Official Symbol 2: B2M
• Synonyms 2: B2M; beta-2-microglobulin; beta-2-microglobin; beta chain of MHC class I molecules
• mRNA Refseq 2: NM_004048
• Protein Refseq 2: NP_004039
• MIM 2: 109700
• UniProt ID 2: P61769