Recombinant Human HLA-B protein, His-tagged

• Cat.No.: HLA-B-8392H
• Product Overview: Recombinant Human HLA-B protein(NP_005505.2), fused to His-tag, was expressed in HEK293.

Specification

• Species: Human
• Source: HEK293
• Tag: His
• Description: HLA-B belongs to the HLA class I heavy chain paralogues. This class I molecule is a heterodimer consisting of a heavy chain and a light chain (beta-2 microglobulin). The heavy chain is anchored in the membrane. Class I molecules play a central role in the immune system by presenting peptides derived from the endoplasmic reticulum lumen. They are expressed in nearly all cells. The heavy chain is approximately 45 kDa and its gene contains 8 exons. Exon 1 encodes the leader peptide, exon 2 and 3 encode the alpha1 and alpha2 domains, which both bind the peptide, exon 4 encodes the alpha3 domain, exon 5 encodes the transmembrane region and exons 6 and 7 encode the cytoplasmic tail. Polymorphisms within exon 2 and exon 3 are responsible for the peptide binding specificity of each class one molecule. Typing for these polymorphisms is routinely done for bone marrow and kidney transplantation. Hundreds of HLA-B alleles have been described.
• Form: Supplied as a 0.2 μm filtered solution in PBS, pH 7.4.
• Molecular Mass: The protein has a calculated MW of 33.6 kDa.
• AA Sequence: HHHHHHGSHSMRYFYTSVSRPGRGEPRFISVGYVDDTQFVRFDSDAASPREEPRAPWIEQEGPEYWDRNTQIYKAQAQTDRESLRNLRGYYNQSEAGSHTLQSMYGCDVGPDGRLLRGHDQYAYDGKDYIALNEDLRSWTAADTAAQITQRKWEAAREAEQRRAYLEGECVEWLRRYLENGKDKLERADPPKTHVTHHPISDHEATLRCWALGFYPAEITLTWQRDGEDQTQDTELVETRPAGDRTFQKWAAVVVPSGEEQRYTCHVQHEGLPKPLTLRWEPSSQSTVPI
• Purity: >75%, by SDS-PAGE
• Storage: Store it under sterile conditions at -20 to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Concentration: 0.5 mg/ml

Gene Information

• Gene Name: HLA-B major histocompatibility complex, class I, B [ Homo sapiens (human) ]
• Official Symbol: HLA-B
• Synonyms: AS; HLAB; B-4901
• Gene ID: 3106
• mRNA Refseq: NM_005514.8
• Protein Refseq: NP_005505.2
• MIM: 142830
• UniProt ID: P01889