Recombinant Human HLA-A*02:01&B2M&WT-1 (RMFPNAPYL) Tetramer protein, His-Avi-tagged, Biotinylated

• Cat.No.: HLA-A&B2M&WT-1-4232H
• Product Overview: Recombinant Human WT-1 (HLA-A*02:01) Tetramer Protein(Gly25-Thr305(HLA-A*02:01), Ile21-Met119(B2M) and RMFPNAPYL peptide) is expressed from HEK293 with His tag and Avi tag at the C-terminal, tetramer is assembled by Biotinylated monomer and streptavidin.

Specification

• Species: Human
• Source: HEK293
• Tag: His&Avi
• ProteinLength: Gly25-Thr305(HLA-A*02:01), Ile21-Met119(B2M)and RMFPNAPYL peptide
• Tag: C-His-Avi
• Conjugation/Label: Biotin
• Form: Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
• Bio-activity: Immobilized Human HLA-A*02:01&B2M&WT-1 (RMFPNAPYL) Tetramer, His Tag at 1μg/ml (100μl/Well) on the plate. Dose response curve for Anti-HLA-A*02:01&B2M&WT-1 (RMFPNAPYL) Antibody, hFc Tag with the EC50 of 14.2ng/ml determined by ELISA.
• Molecular Mass: The protein has a predicted MW of 258 kDa. Due to glycosylation, the protein migrates to 260-265 kDa under Non reducing (N) condition based on Tris-Bis PAGE result.
• Endotoxin: Less than 1EU per μg by the LAL method.
• Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
• Storage: Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
• Reconstitution: Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
• GeneID 2: 567

Gene Information

• Gene Name: HLA-A major histocompatibility complex, class I, A [ Homo sapiens ]
• Official Symbol: HLA-A
• Synonyms: HLA-A; major histocompatibility complex, class I, A; HLA class I histocompatibility antigen, A-1 alpha chain; antigen presenting molecule; leukocyte antigen class I-A; MHC class I antigen HLA-A heavy chain; HLAA; FLJ26655
• Gene ID: 3105
• mRNA Refseq: NM_001242758
• Protein Refseq: NP_001229687
• UniProt ID: P01891
• Gene Name 2: B2M beta-2-microglobulin [ Homo sapiens ]
• Official Symbol 2: B2M
• Synonyms 2: B2M; beta-2-microglobulin; beta-2-microglobin; beta chain of MHC class I molecules
• mRNA Refseq 2: NM_004048
• Protein Refseq 2: NP_004039
• MIM 2: 109700
• UniProt ID 2: P61769