Recombinant Human HLA-A*02:01&B2M&MAGE-A4 protein

• Cat.No.: HLA-A*02:01&B2M&MAGE-A4-1548H
• Product Overview: Recombinant Human HLA-A*02:01&B2M&MAGE-A4 protein(Gly25-Ile308(HLA-A*02:01)&Ile21-Met119(B2M)&KVLEHVVRV) was expressed in HEK293.

Specification

• Species: Human
• Source: HEK293
• Tag: Non
• ProteinLength: 25-308;21-119 a.a.
• Form: Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
• Molecular Mass: Molecular CharacterizationHuman HLA-A*02:01&B2M&MAGE-A4 (KVLEHVVRV) Tetramer Protein is assembled by biotinylated monomer and streptavidin.Biotinylated Human HLA-A*02:01&B2M&MAGE-A4 (KVLEHVVRV) Complex Protein is produced by co-expression of HLA and B2M loaded with MAGE-A4 peptide. Biotinylated Human HLA-A*02:01&B2M&MAGE-A4 (KVLEHVVRV) Complex Protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™).The protein has a calculated MW of 36.3 kDa, 13.3 kDa and 11.7 kDa. The protein migrates as 40-45 kDa, 15 kDa and 13 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Proteinlength: Gly25-Ile308(HLA-A*02:01)&Ile21-Met119(B2M)&KVLEHVVRV
• Endotoxin: Less than 1.0 EU per μg by the LAL method.
• Purity: >95% as determined by SDS-PAGE. >90% as determined by SEC-MALS.
• Storage: For long term, the product should be stored at lyophilized state at -20°C or lower.Please avoid repeated freeze-thaw cycles. This product is stable after at:
  -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: HLA-A major histocompatibility complex, class I, A [ Homo sapiens ]
• Official Symbol: HLA-A
• Synonyms: HLA-A; major histocompatibility complex, class I, A; HLA class I histocompatibility antigen, A-1 alpha chain; antigen presenting molecule; leukocyte antigen class I-A; MHC class I antigen HLA-A heavy chain; HLAA; FLJ26655
• Gene ID: 3105
• mRNA Refseq: NM_001242758
• Protein Refseq: NP_001229687
• UniProt ID: P01891