Recombinant Human HIST1H2AJ protein, His & GST-tagged

• Cat.No.: HIST1H2AJ-1519H
• Product Overview: Recombinant Human HIST1H2AJ aa. (Met1~Lys128 (Accession # Q99878)) fused with N-terminal His & GST tag was produced in E. coli cells.

Specification

• Description: Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H2A family. Transcripts from this gene lack polyA tails but instead contain a palindromic termination element. This gene is found in the small histone gene cluster on chromosome 6p22-p21.3.
• Source: E. coli
• Species: Human
• Tag: His&GST
• Form: Freeze-dried powder
• Molecular Mass: Predicted Molecular Mass: 41.0kDa
• Protein length: Met1~Lys128
• Endotoxin: <1.0EU per 1ug (determined by the LAL method)
• Purity: >95%
• Applications: SDS-PAGE; WB; ELISA; IP.
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
• Storage buffer: Supplied as lyophilized form in PBS, pH7.4, containing 5% sucrose, 0.01% sarcosyl.
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Isoelectric Point: 9.4

Gene Information

• Gene Name: HIST1H2AJ histone cluster 1 H2A family member j [ Homo sapiens (human) ]
• Official Symbol: HIST1H2AJ
• Synonyms: HIST1H2AJ; histone cluster 1 H2A family member j; H2A/E; H2AFE; dJ160A22.4; histone cluster 1, H2aj; H2A histone family, member E; histone 1, H2aj
• Gene ID: 8331
• mRNA Refseq: NM_021066.2
• Protein Refseq: NP_066544.1
• UniProt ID: Q99878