Recombinant Human HIST1H2AD protein, His & GST-tagged

• Cat.No.: HIST1H2AD-1518H
• Product Overview: Recombinant Human HIST1H2AD aa. (Ser2~Lys130 (Accession # P20671)) fused with N-terminal His & GST tag was produced in E. coli cells.

Specification

• Description: Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around a nucleosome, an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H2A family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6p22-p21.3.
• Source: E. coli
• Species: Human
• Tag: His&GST
• Form: Freeze-dried powder
• Molecular Mass: Predicted Molecular Mass: 41.0kDa
• Protein length: Ser2~Lys130
• Endotoxin: <1.0EU per 1ug (determined by the LAL method)
• Purity: >95%
• Applications: SDS-PAGE; WB; ELISA; IP.
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
• Storage buffer: Supplied as lyophilized form in PBS, pH7.4, containing 5% sucrose, 0.01% sarcosyl.
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Isoelectric Point: 9.4

Gene Information

• Gene Name: HIST1H2AD histone cluster 1 H2A family member d [ Homo sapiens (human) ]
• Official Symbol: HIST1H2AD
• Synonyms: HIST1H2AD; histone cluster 1 H2A family member d; H2A.3; H2A/g; H2AFG; histone H2A type 1-D; H2A histone family, member G; histone 1, H2ad; histone H2A.3; histone H2A/g; histone H2AD; histone cluster 1, H2ad
• Gene ID: 3013
• mRNA Refseq: NM_021065.3
• Protein Refseq: NP_066409.1
• UniProt ID: P20671