Recombinant Human HGF Protein, None-tagged, Alexa Fluor 488 conjugated

• Cat.No.: HGF-872HAF488
• Product Overview: Alexa Fluor 488 conjugated recombinant human HGF (NP_000592.3) precursor (Met 1-Ser 728), fused with no tag, was produced in CHO Stable Cells.

Specification

• Species: Human
• Source: CHO
• Tag: Non
• Protein Length: 697
• Form: Lyophilized
• Molecular Mass: The secreted recombinant human HGF consists of 697 amino acids after cleavage of the signal peptide and has a predicted molecular mass of 79.7 kDa. The HGF single chain can be processed into the active form of disulfide-linked heterodimer of α and β chain. As a result of glycosylation, it migrates with the apparent molecular mass of 90, 60 and 34 kDa corresponding to the single chain, α chain and β chain respectively in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 EU/ μg of the protein as determined by the LAL method.
• Characteristic: Disulfide-linked homodimer
  Labeled with Alexa Fluor 488 via amines
  Excitation Wavelength: 488 nm
  Emission Wavelength: 515­-545 nm
• Stability: Samples are stable for up to 12 months from date of receipt at -70 centigrade.
• Storage: Store it under sterile conditions at -20 to -70 centigrade. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Storage Buffer: Lyophilized from sterile PBS, pH 7.4
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution. Centrifuge the vial at 4 centigrade before opening to recover the entire contents.

Gene Information

• Gene Name: HGF hepatocyte growth factor (hepapoietin A; scatter factor) [ Homo sapiens ]
• Official Symbol: HGF
• Gene ID: 3082
• mRNA Refseq: NM_000601
• Protein Refseq: NP_000592
• MIM: 142409
• UniProt ID: P14210