Recombinant Human HGF, His-tagged

• Cat.No.: HGF-593H
• Product Overview: Recombinant Human HGF Protein(Met1-Ser728), made in HEK293, with C-His Tag.

Specification

• Species: Human
• Source: HEK293
• Tag: His
• Protein Length: 1-728 a.a.
• Description: Hepatocyte growth factor regulates cell growth, cell motility, and morphogenesis by activating a tyrosine kinase signaling cascade after binding to the proto-oncogenic c-Met receptor. Hepatocyte growth factor is secreted by mesenchymal cells and acts as a multi-functional cytokine on cells of mainly epithelial origin. Its ability to stimulate mitogenesis, cell motility, and matrix invasion gives it a central role in angiogenesis, tumorogenesis, and tissue regeneration. It is secreted as a single inactive polypeptide and is cleaved by serine proteases into a 69-kDa alpha-chain and 34-kDa beta-chain. A disulfide bond between the alpha and beta chains produces the active, heterodimeric molecule. The protein belongs to the plasminogen subfamily of S1 peptidases but has no detectable protease activity. Alternative splicing of this gene produces multiple transcript variants encoding different isoforms.
• Form: Protein in PBS (with 5% trehalose and mannitol) was 0.22 µm filtered before lyophilized.
• Endotoxin: Less than 1.0 EU/µg by LAL method
• Purity: > 95% as determined by SDS-PAGE
• Storage: Lyophilized form is stable at room temperature for 3 weeks. Keep desiccated at below -18°C for long term storage. After reconstituted, store at 4°C for 2-7 days or at below -18°C for up to 3 months.
• Reconstitution: Reconstitute lyophilized powder with water to no less than 100 µg/ml, which can be further diluted in other desired aqueous solutions.

Gene Information

• Gene Name: HGF hepatocyte growth factor (hepapoietin A; scatter factor) [ Homo sapiens (human) ]
• Official Symbol: HGF
• Synonyms: HGF; SF; HGFB; HPTA; F-TCF; DFNB39; hepatocyte growth factor (hepapoietin A; scatter factor); hepatocyte growth factor; hepatopoeitin-A; hepatopoietin-A; lung fibroblast-derived mitogen; fibroblast-derived tumor cytotoxic factor
• Gene ID: 3082
• mRNA Refseq: NM_000601
• Protein Refseq: NP_000592
• MIM: 142409
• UniProt ID: P14210
• Chromosome Location: 7q21.1
• Pathway: Arf6 signaling events; Cytokine Signaling in Immune system; Cytokine-cytokine receptor interaction
• Function: chemoattractant activity; growth factor activity; identical protein binding