Recombinant Human heterogeneous nuclear ribonucleoprotein A/B, His-tagged

• Cat.No.: HNRNPAB-288H
• Product Overview: HNRNPAB, 1-285aa, Human, His tag, E.coli

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Protein Length: 1-285 a.a.
• Description: HNRNPAB belongs to the subfamily of ubiquitously expressed heterogeneous nuclear ribonucleoproteins (hnRNPs). The hnRNPs are produced by RNA polymerase II and are components of the heterogeneous nuclear RNA (hnRNA) complexes. They are associated with pre-mRNAs in the nucleus and appear to influence pre-mRNA processing and other aspects of mRNA metabolism and transport. While all of the hnRNPs are present in the nucleus, some seem to shuttle between the nucleus and the cytoplasm. The hnRNP proteins have distinct nucleic acid binding properties. HNRNPAB, which binds to one of the components of the multiprotein editosome complex, has two repeats of quasi-RRM (RNA recognition motif) domains that bind to RNAs. Recombinant human HNRNPAB protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
• Form: Liquid. In PBS containing 30% glycerol, 1mM DTT
• Molecular Mass: 33 kDa (308aa) confirmed by MALDI-TOF
• AA Sequence: MGSSHHHHHH SSGLVPRGSH MGSMSEAGEE QPMETTGATE NGHEAVPEGE SPAGAGTGAA AGAGGATAAP PSGNQNGAEG DQINASKNEE DAGKMFVGGL SWDTSKKDLK DYFTKFGEVV DCTIKMDPNT GRSRGFGFIL FKDAASVEKV LDQKEHRLDG RVIDPKKAMA MKKDPVKKIF VGGLNPEATE EKIREYFGEF GEIEAIELPM DPKLNKRRGF VFITFKEEEP VKKVLEKKFH TVSGSKCEIK VAQPKEVYQQ QQYGSGGRGN RNRGNRGSGG GGGGGGQGST NYGKSQRRGG HQNNYKPY
• Purity: >95% by SDS - PAGE
• Storage: Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
• Concentration: 0.25mg/ml (determined by Bradford assay)

Gene Information

• Gene Name: HNRNPAB heterogeneous nuclear ribonucleoprotein A/B [ Homo sapiens ]
• Official Symbol: HNRNPAB
• Synonyms: ABBP1; HNRPAB
• Gene ID: 3182
• mRNA Refseq: NM_031266.2
• Protein Refseq: NP_112556.2
• MIM: 602688
• UniProt ID: Q99729
• Chromosome Location: 5q35.3
• Pathway: mRNA processing, organism-specific biosystem
• Function: RNA binding;mRNA binding;nucleotide binding;contributes_to sequence-specific DNA binding;contributes_to sequence-specific DNA binding transcription factor activity