Recombinant Human Heparin-Binding EGF-Like Growth Factor

• Cat.No.: HBEGF-339H

Specification

• Species: Human
• Source: Sf21 Cells
• Tag: Non
• Description: Heparin-binding EGF-like growth factor (HB-EGF) is a monomeric heparin binding O-glycosylated protein and a member of the EGF family of proteins. It has been shown to play a role in wound healing, cardiac hypertrophy and heart development and function. HB-EGF contributes to cell adhesion, invasion, and angiogenesis, which are integral to transcoelomic metastasis in ovarian cancer.
• Molecular Weight: The predicted molecular weight of Recombinant Human HB-EGF is 9.5 kDa. However, this recombinant protein is heterogenously O-glycosylated and the actual molecular weight as observed by migration on SDS Page is 12 kDa.
• Formulation: This recombinant protein was 0.2 µm filtered and lyophilized from modified Dulbecco's phosphate buffered saline (1X PBS) pH 7.2 – 7.3 with no calcium, magnesium, or preservatives present.
• State Of Matter: Lyophilized.
• Purity: >97% by SDS Page and analyzed by silver stain.
• Endotoxin: <1.0 EU/µg as determined by the LAL method.
• Biological Activity: The biological activity of Human HB-EGF was determined by its ability to stimulate proliferation in a mouse fibroblast cell line, Balb/3T3. The expected ED50 for this effect is typically 0.15 - 0.75 ng/mL. The cell number is assessed in a fluorometric assay using the redox sensitive dye, Resazurin.
• Storage: This lyophilized protein is stable for six to twelve months when stored desiccated at -20°Cto -70°C. After aseptic reconstitution, this protein may be stored at 2°Cto 8°Cfor one month or at -20°Cto -70°Cin a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. See Product Insert for exact lot specific storage instructions.

Gene Information

• Gene Name: HBEGF heparin-binding EGF-like growth factor [ Homo sapiens ]
• Synonyms: HBEGF; heparin-binding EGF-like growth factor; DTR; DTS; DTSF; HEGFL; diphtheria toxin receptor (heparin-binding EGF-like growth factor); diphtheria toxin receptor (heparin-binding epidermal growth factor-like growth factor); heparin-binding epidermal growth factor
• Gene ID: 1839
• mRNA Refseq: NM_001945
• Protein Refseq: NP_001936
• MIM: 126150
• UniProt ID: Q99075
• Chromosome Location: 5q23
• Pathway: Epithelial cell signaling in Helicobacter pylori infection; ErbB signaling pathway; GnRH signaling pathway
• Function: epidermal growth factor receptor binding; growth factor activity; heparin binding; receptor activity