Recombinant Human Heat Shock 70kDa Protein 5 (Glucose-regulated Protein, 78kDa), His-tagged

• Cat.No.: HSPA5-332H
• Product Overview: Recombinant humanHSPA5 protein, fused to His-tag at C-terminus, was expressed in E.coli and purified by using conventional chromatography techniques (640 aa, 71 kDa).

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Description: Binding immunoglobulin protein (BiP)belongs to the family of ~70 kDa heat shock proteins (HSP 70). It is a stress response protein which is induced by agents or conditions that adversely affect endoplasmic reticulum (ER) function. This protein is essential for the proper glycosylation, folding as well as for the maintenance of cell homeostasis and the prevention of apoptosis.
• Formulation: Liquid. In 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol.
• Molecular Weight: 71 kDa (640 aa).
• Purity: > 90% by SDS – PAGE.
• Concentration: 1 mg/ml (determined by Bradford assay).
• Sequence: MEEDKKEDVG TVVGIDLGTT YSCVGVFKNG RVEIIANDQG NRITPSYVAF TPEGERLIGD AAKNQLTSNP ENTVFDAKRL IGRTWNDPSV QQDIKFLPFK VVEKKTKPYI QVDIGGGQTK TFAPEEISAM VLTKMKETAE AYLGKKVTHA VVTVPAYFND AQRQATKDAG TIAGLNVMRI INEPTAAAIA YGLDKREGEK NILVFDLGGG TFDVSLLTID NGVFEVVATN GDTHLGGEDF DQRVMEHFIK LYKKKTGKDV RKDNRAVQKL RREVEKAKRA LSSQHQARIE IESFYEGEDF SETLTRAKFE ELNMDLFRST MKPVQKVLED SDLKKSDIDE IVLVGGSTRI PKIQQLVKEF FNGKEPSRGI NPDEAVAYGA AVQAGVLSGD QDTGDLVLLD VCPLTLGIET VGGVMTKLIP RNTVVPTKKS QIFSTASDNQ PTVTIKVYEG ERPLTKDNHL LGTFDLTGIP PAPRGVPQIE VTFEIDVNGI LRVTAEDKGT GNKNKITITN DQNRLTPEEI ERMVNDAEKF AEEDKKLKER IDTRNELESY AYSLKNQIGD KEKLGGKLSS EDKETMEKAV EEKIEWLESH QDADIEDFKA KKKELEEIVQ PIISKLYGSA GPPPTGEEDT AELEHHHHHH
• Storage: Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.

Gene Information

• Gene Name: HSPA5
• Synonyms: HSPA5; heat shock 70kDa protein 5 (glucose-regulated protein, 78kDa); HSPA5 heat shock 70kDa protein 5 (glucose-regulated protein, 78kDa); BIP; MIF2; GRP78; GRP 78; FLJ26106; BiP; OTTHUMP00000022124; Heat shock 70 kDa protein 5; Endoplasmic reticulum lumenal Ca(2+)-binding protein grp78; Heat-shock 70kD protein-5 (glucose-regulated protein, 78kD); Immunoglobulin heavy chain-binding protein; heat shock 70kD protein 5 (glucose-regulated protein, 78kD); heat shock 70kDa protein 5.
• Gene ID: 3309
• mRNA Refseq: NM_005347.3
• Protein Refseq: NP_005338.1
• MIM: 138120
• UniProt ID: P11021
• Chromosome Location: 9q33-q34.1
• Pathway: Antigen processing and presentation; Neurodegenerative Disorders; Prion disease; Hemostasis.
• Function: ATP binding; calcium ion binding; caspase inhibitor activity; nucleotide binding; protein binding, bridging; ribosome binding; unfolded protein binding.