Recombinant Human HDAC6 protein, His-tagged

• Cat.No.: HDAC6-131H
• Product Overview: Recombinant Human HDAC6(Val166~Leu348) fused with His tag at N-terminal was expressed in E. coli.

Specification

• Description: Histones play a critical role in transcriptional regulation, cell cycle progression, and developmental events. Histone acetylation/deacetylation alters chromosome structure and affects transcription factor access to DNA. The protein encoded by this gene belongs to class II of the histone deacetylase/acuc/apha family. It contains an internal duplication of two catalytic domains which appear to function independently of each other. This protein possesses histone deacetylase activity and represses transcription.
• Source: E. coli
• Species: Human
• Tag: His
• Form: PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300
• Molecular Mass: 22.0kDa
• Protein length: Val166~Leu348
• Endotoxin: <1.0EU per 1µg (determined by the LAL method)
• Purity: > 95%
• Applications: Positive Control; Immunogen; SDS-PAGE; WB.
  If bio-activity of the protein is needed, please check active protein
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 centigrade for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 centigrade for one month. Aliquot and store at -80 centigrade for 12 months.
• Reconstitution: Reconstitute in PBS or others.

Gene Information

• Gene Name: HDAC6 histone deacetylase 6 [ Homo sapiens ]
• Official Symbol: HDAC6
• Synonyms: HDAC6; histone deacetylase 6; FLJ16239; HD6; JM21; KIAA0901
• Gene ID: 10013
• mRNA Refseq: NM_006044
• Protein Refseq: NP_006035
• MIM: 300272
• UniProt ID: Q9UBN7
• Chromosome Location: Xp11.23
• Pathway: Cell cycle, organism-specific biosystem; NOTCH1 Intracellular Domain Regulates Transcription, organism-specific biosystem; Signal Transduction, organism-specific biosystem; Signaling by NOTCH, organism-specific biosystem; Signaling by NOTCH1, organism-specific biosystem; Signaling events mediated by HDAC Class I, organism-specific biosystem; Signaling events mediated by HDAC Class II, organism-specific biosystem
• Function: Hsp90 protein binding; NAD-dependent histone deacetylase activity (H3-K14 specific); NAD-dependent histone deacetylase activity (H3-K9 specific); NAD-dependent histone deacetylase activity (H4-K16 specific); actin binding; alpha-tubulin binding; beta-catenin binding; beta-tubulin binding; dynein complex binding; enzyme binding; histone deacetylase activity; histone deacetylase activity (H3-K16 specific); histone deacetylase binding; hydrolase activity; metal ion binding; microtubule binding; microtubule binding; polyubiquitin binding; protein binding; tau protein binding; tubulin deacetylase activity; tubulin deacetylase activity; zinc ion binding