Recombinant Human HDAC1 protein, His-tagged

• Cat.No.: HDAC1-550H
• Product Overview: Recombinant Human HDAC1 protein(Met1~Ala482), fused with N-terminal His tag, was expressed in E. coli.

Specification

• Source: E. coli
• Species: Human
• Tag: His
• Protein length: Met1~Ala482
• Form: 20mM Tris, 150mM NaCl, pH8.0, containing 0.05% sarcosyl and 5% trehalose.
• Bio-activity: Histone deacetylase 1 (HDAC1) is an enzyme which belongs to the histone deacetylase/acuc/apha family and is a component of the histone deacetylase complex. Histone acetylation and deacetylation, catalyzed by multisubunit complexes, play a key role in the regulation of eukaryotic gene expression. HDAC1 also interacts with retinoblastoma tumor-suppressor protein and this complex is a key element in the control of cell proliferation and differentiation. Besides, Specificity Protein 1 (Sp1) has been identified as an interactor of HDAC1, thus a binding ELISA assay was conducted to detect the interaction of recombinant human HDAC1 and recombinant human Sp1. Briefly, HDAC1 were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100uL were then transferred to Sp1-coated microtiter wells and incubated for 2h at 37°C. Wells were washed with PBST and incubated for 1h with anti-HDAC1 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 °C . Finally, add 50μL stop solution to the wells and read at 450nm immediately. The binding activityof of
  HDAC1 and Sp1 was shown in Figure, and this effect was in a dose dependent manner.
• Molecular Mass: 70kDa
• Purity: > 95%
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
• Reconstitution: Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Gene Information

• Gene Name: HDAC1 histone deacetylase 1 [ Homo sapiens ]
• Official Symbol: HDAC1
• Synonyms: HDAC1; histone deacetylase 1; RPD3L1; GON 10; HD1; reduced potassium dependency, yeast homolog-like 1; RPD3; GON-10; DKFZp686H12203
• Gene ID: 3065
• mRNA Refseq: NM_004964
• Protein Refseq: NP_004955
• MIM: 601241
• UniProt ID: Q13547

Reviews

03/12/2022

The HDAC1 proteins purchased from your company is suitable for ELISA.

09/24/2021

The shipping was prompt enough for me to get into the experiment in the shortest time possible.

12/01/2020

Friendly customer service and detailed answers to all the questions I asked!

Q&As

What diseases has HDAC1 been associated with the study of?

HDAC1 is closely related to the study of diseases such as lung cancer and esophageal squamous carcinoma.

What is the relationship between HDAC1 and Adriamycin?

Adriamycin specifically reduces HDAC1 protein expression.

How is HDAC1 expressed in esophageal squamous carcinoma tissues?

HDAC1 protein is highly expressed in esophageal squamous carcinoma tissues, and its high expression correlates with the development of esophageal squamous carcinoma.

What biological function does HDAC1 have?

HDAC1 is a key enzyme in the histone deacetylation process and is involved in the regulation of histone acetylation levels.