Recombinant Full Length Mouse HDAC1 Protein, C-Flag,C-His-tagged

• Cat.No.: HDAC1-13MFL
• Product Overview: Recombinant Full Length Mouse HDAC1 Protein, fused to Flag and His-tag at C-terminus, was expressed in Sf9 insect cells.

Specification

• Description: Enables several functions, including E-box binding activity; Krueppel-associated box domain binding activity; and promoter-specific chromatin binding activity. Involved in several processes, including animal organ development; histone deacetylation; and regulation of gene expression. Acts upstream of or within several processes, including negative regulation of signal transduction; negative regulation of transcription by RNA polymerase II; and nervous system development. Located in heterochromatin; neuronal cell body; and nucleus. Part of NuRD complex and transcription repressor complex. Is expressed in several structures, including brain; early conceptus; embryo mesenchyme; genitourinary system; and sensory organ. Orthologous to human HDAC1 (histone deacetylase 1).
• Source: Insect cells
• Species: Mouse
• Tag: C-Flag,C-His
• Protein length: 1-482 aa
• Form: 40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 0.04% Tween20, 20% glycerol, 100ug/ml flag peptide.
• Bio-activity: ≥460 pmol/min/μg.
• Molecular Mass: 57 kDa
• Purity: ≥50%
• Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
• Storage: Store at -80 centigrade.
• Storage Buffer: 25 mM Tris/HCl, pH 8.0, 137 mM NaCl, 2.7 mM KCl, 1 mM MgCl2, and 0.1 mg/ml BSA, 20 μM BPS HDAC substrate 3, and Mouse HDAC1 (0.3-20 ng). Incubation condition: 30 min at 37°C followed by developing for 15 min at room temperature.
• Use/Stability: Thaw on ice and gently mix prior to use. DO NOT VORTEX. Perform a quick spin before opening. Aliquot into small volumes and flash freeze for long term storage. Avoid multiple freeze/thaw cycles.
• References: 1. Rikimaru, T. et al., Oncology 72(1-2), 69-74 (2007).
  2. Ishihama, K. et al., J Clin Pathol 60(11), 1205-10 (2007).

Gene Information

• Gene Name: Hdac1 histone deacetylase 1 [ Mus musculus (house mouse) ]
• Official Symbol: HDAC1
• Synonyms: HDAC1; HD1; RPD3; MommeD5; Hdac1-ps; histone deacetylase 1; HDAC-1; HDAC1 Tag
• Gene ID: 433759
• mRNA Refseq: NM_008228.3
• Protein Refseq: NP_032254.1
• UniProt ID: O09106