Recombinant Human HBD protein, His & GST-tagged

• Cat.No.: HBD-7861H
• Product Overview: Recombinant Human HBD aa. (Met1~Ala143 (Accession # P02042)) fused with N-terminal His & GST tag was produced in E. coli cells.

Specification

• Description: The delta (HBD) and beta (HBB) genes are normally expressed in the adult: two alpha chains plus two beta chains constitute HbA, which in normal adult life comprises about 97% of the total hemoglobin. Two alpha chains plus two delta chains constitute HbA-2, which with HbF comprises the remaining 3% of adult hemoglobin. Five beta-like globin genes are found within a 45 kb cluster on chromosome 11 in the following order: 5'-epsilon--Ggamma--Agamma--delta--beta-3'. Mutations in the delta-globin gene are associated with beta-thalassemia.
• Source: E. coli
• Species: Human
• Tag: His&GST
• Form: Freeze-dried powder
• Molecular Mass: Predicted Molecular Mass: 45.5kDa
• Protein length: Met1~Ala143
• Endotoxin: <1.0EU per 1ug (determined by the LAL method)
• Purity: >95%
• Characteristic: The isoelectric point is 7.
• Applications: SDS-PAGE; WB; ELISA; IP
• Stability: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
• Storage Buffer: Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl.
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.

Gene Information

• Gene Name: HBD hemoglobin subunit delta [ Homo sapiens (human) ]
• Official Symbol: HBD
• Synonyms: HBD; hemoglobin subunit delta; delta globin; delta-globin chain; hemoglobin delta chain; hemoglobin, delta
• Gene ID: 3045
• mRNA Refseq: NM_000519.3
• Protein Refseq: NP_000510.1
• UniProt ID: P02042