Recombinant Human HARS2 Protein, His-tagged

• Cat.No.: HARS2-2228H
• Product Overview: Recombinant Human HARS2 Protein (Lys231-Ser506) with a N-His tag was expressed in E. coli.

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Protein Length: Lys231-Ser506
• Description: Aminoacyl-tRNA synthetases are a class of enzymes that charge tRNAs with their cognate amino acids. The protein encoded by this gene is an enzyme belonging to the class II family of aminoacyl-tRNA synthetases. Functioning in the synthesis of histidyl-transfer RNA, the enzyme plays an accessory role in the regulation of protein biosynthesis. The gene is located in a head-to-head orientation with HARS on chromosome five, where the homologous genes likely share a bidirectional promoter. Mutations in this gene are associated with the pathogenesis of Perrault syndrome, which involves ovarian dysgenesis and sensorineural hearing loss. Alternative splicing results in multiple transcript variants of this gene.
• Form: Freeze-dried powder
• Molecular Mass: Predicted Molecular Mass: 34.6 kDa
  Accurate Molecular Mass: 35 kDa
• Endotoxin: <1.0 EU per 1µg (determined by the LAL method).
• Purity: > 90%
• Applications: Positive Control; Immunogen; SDS-PAGE; WB.
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 centigrade for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 centigrade for one month. Aliquot and store at -80 centigrade for 12 months.
• Storage Buffer: PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
• Reconstitution: Reconstitute in PBS or others.

Gene Information

• Gene Name: HARS2 histidyl-tRNA synthetase 2, mitochondrial (putative) [ Homo sapiens (human) ]
• Official Symbol: HARS2
• Synonyms: HARS2; histidyl-tRNA synthetase 2, mitochondrial (putative); HARSL, histidyl tRNA synthetase like; probable histidine--tRNA ligase, mitochondrial; HARSR; histidine tRNA ligase 2; mitochondrial (putative); HO3; hisRS; HARS-related; histidine translase; histidine-tRNA ligase homolog; probable histidyl-tRNA synthetase, mitochondrial; histidine tRNA ligase 2, mitochondrial (putative); HARSL
• Gene ID: 23438
• mRNA Refseq: NM_012208
• Protein Refseq: NP_036340
• MIM: 600783
• UniProt ID: P49590