Recombinant Human HARS protein (Ala2-Cys509), His-tagged

• Cat.No.: HARS-370H
• Product Overview: Recombinant human HARS (NP_002100.2) (Ala2-Cys509) protein, fused with a polyhistidine tag at the N-terminus, was expressed in Yeast.

Specification

• CommonName: HARS
• Source: Baculovirus-Insect Cells
• Species: Human
• Tag: His
• Predicted N Terminal: His
• Form: Lyophilized from sterile 20 mM Tris, 200 mM NaCl, pH 7.5, 20 % glycerol, 5 % trehalose, 5% mannitol and 0.01% Tween80.
• Molecular Mass: The recombinant human HARS consists of 527 amino acids and predicts a molecular mass of 59.6 kDa.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
• Purity: > 90 % as determined by SDS-PAGE.
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.25 mg/ml. Centrifuge the vial at 4°C before opening to recover the entire contents.
• Shipping: In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
• Protein length: 2-509 a.a.

Gene Information

• Gene Name: HARS histidyl-tRNA synthetase [ Homo sapiens ]
• Official Symbol: HARS
• Synonyms: HARS; histidyl-tRNA synthetase; histidine--tRNA ligase, cytoplasmic; histidine tRNA ligase 1; cytoplasmic; HisRS; histidine translase; HRS; USH3B; FLJ20491
• Gene ID: 3035
• mRNA Refseq: NM_001258040
• Protein Refseq: NP_001244969
• MIM: 142810
• UniProt ID: P12081