Recombinant Human H3 histone, family 3A, His-tagged

• Cat.No.: H3F3A-568H
• Product Overview: H3F3A, 1-136aa, Human, His tag, E.coli

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Protein Length: 1-136 a.a.
• Description: Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. H3F3A is a replication-independent member of the histone H3 family. Recombinant human H3F3A protein, fused to His-tag at N-terminus, was expressed in E.coli.
• Form: Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 0.4M urea, 10% glycerol
• Molecular Mass: >90% by SDS - PAGE
• AA Sequence: MGSSHHHHHH SSGLVPRGSH MGSMARTKQT ARKSTGGKAP RKQLATKAAR KSAPSTGGVK KPHRYRPGTV ALREIRRYQK STELLIRKLP FQRLVREIAQ DFKTDLRFQS AAIGALQEAS EAYLVGLFED TNLCAIHAKR VTIMPKDIQL ARRIRGERA
• Purity: >90% by SDS - PAGE
• Storage: Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
• Concentration: 1 mg/ml (determined by Bradford assay)

Gene Information

• Gene Name: H3F3A H3 histone, family 3A [ Homo sapiens ]
• Official Symbol: H3F3A
• Synonyms: H3F3A; H3 histone, family 3A; H3F3; histone H3.3; H3.3A; H3F3B; MGC87782; MGC87783
• Gene ID: 3020
• mRNA Refseq: NM_002107
• Protein Refseq: NP_002098
• MIM: 601128
• UniProt ID: P84243
• Chromosome Location: 1q42.12
• Pathway: Alcoholism, organism-specific biosystem; Alcoholism, conserved biosystem; Amyloids, organism-specific biosystem; Disease, organism-specific biosystem; Factors involved in megakaryocyte development and platelet production, organism-specific biosystem; Gene Expression, organism-specific biosystem; Hemostasis, organism-specific biosystem