Recombinant Human Guanylate Cyclase Activator 2A

• Cat.No.: GUCA2A-86H
• Product Overview: Proguanylin Human Recombinant produced inE.coli, is an 11kDa protein containing 96 amino acid residues of the human proguanylin and 2 additional amino acid residues (bold). The Proguanylin is purified by proprietary chromatographic techniques.

Specification

• Species: Human
• Source: E.coli
• Tag: Non
• Description: Heat-stable enterotoxins (STa) are small, cysteine-rich peptides secreted by Escherichia coli that are able to induce diarrhea through the stimulation of an intestine-specific receptor-guanylyl cyclase known as STaR. Binding of STa to STaR induces a dramatic increase in the cGMP content of the cell; the increase, in turn, inhibits salt absorption and stimulates chloride secretion. This imbalance of ions is accompanied by a massive accumulation of water in the gut that gives rise to the diarrhea and dehydration characteristic of enterotoxin activity. The hormonal form of guanylin is a 94-amino acid peptide with a molecular mass of 10.3kDa. Guanylin is synthesized by gut enterochromaffin cells as a prohormone of 115 amino acids and is processed to the molecular form of 94 amino acids circulating in the blood. GPVTVQDGNF SFSLESVKKL KDLQEPQEPR VGKLRNFAPI PGEPVVPILC SNPNFPEELK PLCKEPNAQE ILQRLEEIAE DPGTCEICAY AACTGC
• Physical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.
• Formulation: Sterile filtered and lyophilized from 0.5 mg/ml in deionized H2O.
• Specificity: The recombinant human proguanylin is 100% homologous with the human proguanylin.
• Purification Method: Three-step procedure using IMAC and size exclusion chromatography before and after refolding.
• Solubility: Add 0.2 ml of deionized H2O and let the lyophilized pellet dissolve completely.
• Applications: Western blotting.
• Purity: Greater than 95.0% as determined by SDS-PAGE.
• Stability: Store lyophilized protein at -20°C. Aliquot the product after reconstitution to avoid repeated freezing/thawing cycles. Reconstituted protein can be stored at 4°C for a limited period of time; it does not show any change after two weeks at 4°C. The lyophilized protein remains stable until the expiry date when stored at -20°C.

Gene Information

• Gene Name: GUCA2A guanylate cyclase activator 2A (guanylin) [ Homo sapiens ]
• Synonyms: GUCA2A; guanylate cyclase activator 2A (guanylin); GUCA2; STARA; GUANYLIN; Gap-I; Guanylin; Guanylate cyclase activator 2A; Guanylate cyclase-activating protein1; HMW-guanylin; OTTHUMP00000007926; guanylate cyclase activator 2A (guanylin 2, intestinal, heat-stable)
• Gene ID: 2980
• mRNA Refseq: NM_033553
• Protein Refseq: NP_291031
• MIM: 139392
• UniProt ID: Q02747
• Chromosome Location: 1p35-p34
• Function: guanylate cyclase activator activity; hormone activity