Recombinant Human GRPR protein, His & T7-tagged

• Cat.No.: GRPR-7835H
• Product Overview: Recombinant Human GRPR aa. (Met1~Lys114 (Accession # P30550)) fused with N-terminal His & T7 tag was produced in E. coli cells.

Specification

• Species: Human
• Source: E.coli
• Tag: His&T7
• Protein Length: Met1~Lys114
• Description: Gastrin-releasing peptide (GRP) regulates numerous functions of the gastrointestinal and central nervous systems, including release of gastrointestinal hormones, smooth muscle cell contraction, and epithelial cell proliferation and is a potent mitogen for neoplastic tissues. The effects of GRP are mediated through the gastrin-releasing peptide receptor. This receptor is a glycosylated, 7-transmembrane G-protein coupled receptor that activates the phospholipase C signaling pathway. The receptor is aberrantly expressed in numerous cancers such as those of the lung, colon, and prostate. An individual with autism and multiple exostoses was found to have a balanced translocation between chromosome 8 and a chromosome X breakpoint located within the gastrin-releasing peptide receptor gene.
• Form: Freeze-dried powder
• Molecular Mass: Predicted Molecular Mass: 16.3kDa.
• Endotoxin: <1.0EU per 1ug (determined by the LAL method)
• Purity: >95%
• Characteristic: The isoelectric point is 6.0.
• Applications: SDS-PAGE; WB; ELISA; IP.
• Stability: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
• Storage Buffer: Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl.
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.

Gene Information

• Gene Name: GRPR gastrin releasing peptide receptor [ Homo sapiens (human) ]
• Official Symbol: GRPR
• Synonyms: PRH2; proline rich protein HaeIII subfamily 2; Pr; pr1/Pr2; PRP-1/PRP-2; salivary acidic proline-rich phosphoprotein 1/2; acidic salivary proline-rich protein, HaeIII type, 2; parotid acidic protein; parotid double-band protein; parotid proline-rich protein 1/2; protein C
• Gene ID: 2925
• mRNA Refseq: NM_005314.2
• Protein Refseq: NP_005305.1
• UniProt ID: P30550