Recombinant Human Granulysin, His-tagged

• Cat.No.: GNLY-4380H
• Product Overview: GNLY Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing double His Tag and having a total molecular mass of 18.1 kDa. The GNLY is purified by proprietary chromatographic techniques.

Specification

• Cat. No.: GNLY-4380H
• Description: GNLY is part of the SAPLIP family and is located in the cytotoxic granules of T cells, which are discharged upon antigen stimulation. GNLY is localized in cytotoxic granules of cytotoxic T lymphocytes and natural killer cells, and it has antimicrobial activity against M. tuberculosis and other organisms. GNLY is an antimicrobial protein that kills intracellular pathogens. GNLY is active against a wide range of microbes, including Gram-positive and Gram-negative bacteria, fungi, and parasites. Kills Mycobacterium tuberculosis.
• Source: Human
• Host: Escherichia Coli.
• Form: The GNLY protein was lyophilized from a concentrated (1mg/ml) solution containing no additives.
• Purity: Greater than 95.0% as determined by SDS-PAGE.
• Physical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.
• Solubility: It is recommended to reconstitute the lyophilized GNLY in sterile 18Mμ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
• Amino acid sequence: The sequence of the first 25 N-terminal amino acids was determined and was found to be MGSSHHHHHHSSGLVPRGSHMMEGL.
• Storage: Lyophilized GNLY although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution GNLY should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.

Gene Information

• Gene Name: GNLY granulysin [ Homo sapiens ]
• Official Symbol: GNLY
• Synonyms: GNLY; granulysin; 519; LAG2; NKG5; LAG-2; D2S69E; TLA519; lymphokine LAG-2; OTTHUMP00000160682; OTTHUMP00000203266; OTTHUMP00000229037; OTTHUMP00000229039; lymphocyte-activation gene 2; T-cell activation protein 519; T-lymphocyte activation gene 519; Protein NKG5
• Gene ID: 10578
• mRNA Refseq: NM_006433
• Protein Refseq: NP_006424
• MIM: 88855
• UniProt ID: P22749
• Chromosome Location: 2p11.2