Recombinant Human GPLD1 Protein, His-tagged

• Cat.No.: GPLD1-172H
• Product Overview: Recombinant Human GPLD1(Thr725~Thr1074) fused with His tag at N-terminal was expressed in E. coli.

Specification

• Description: Many proteins are tethered to the extracellular face of eukaryotic plasma membranes by a glycosylphosphatidylinositol (GPI) anchor. The GPI-anchor is a glycolipid found on many blood cells. The protein encoded by this gene is a GPI degrading enzyme. Glycosylphosphatidylinositol specific phospholipase D1 hydrolyzes the inositol phosphate linkage in proteins anchored by phosphatidylinositol glycans, thereby releasing the attached protein from the plasma membrane.
• Source: E. coli
• Species: Human
• Tag: His
• Form: 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.
• Molecular Mass: 47.1kDa
• Protein length: Thr725~Thr1074
• Endotoxin: <1.0EU per 1ug (determined by the LAL method)
• Purity: > 90%
• Applications: Positive Control; Immunogen; SDS-PAGE; WB.
  If bio-activity of the protein is needed, please check active protein
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 centigrade for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 centigrade for one month. Aliquot and store at -80 centigrade for 12 months.
• Reconstitution: Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Gene Information

• Gene Name: GPLD1 glycosylphosphatidylinositol specific phospholipase D1 [ Homo sapiens ]
• Official Symbol: GPLD1
• Synonyms: GPLD1; glycosylphosphatidylinositol specific phospholipase D1; phosphatidylinositol-glycan-specific phospholipase D; GPI-PLD; PI-G PLD; GPI-specific phospholipase D; glycoprotein phospholipase D; phospholipase D, phosphatidylinositol-glycan-specific; glycosylphosphatidylinositol specific phospholipase D1, isoform 2; GPIPLD; PIGPLD; GPIPLDM; PIGPLD1; MGC22590
• Gene ID: 2822
• mRNA Refseq: NM_001503
• Protein Refseq: NP_001494
• MIM: 602515
• UniProt ID: P80108